牙科粘结剂对人中性粒细胞的影响

Comparison of the effects of three dental cements on polymorph nuclear neutrophils

目的:研究3种固定修复用粘结剂对人中性粒细胞(polymorph nuclear neutrophils,PMNs)相关活性以及致炎性等方面的影响。方法:在24孔板底部铺上玻璃离子水门汀、聚羧酸锌水门汀、光固化树脂水门汀,采集健康成人志愿者静脉血分离提纯PMNs,将PMNs与3种粘结剂接触培养4 h后,检测细胞的早期凋亡率、坏死率以及存活率的变化,产生的活性氧簇(reactiveoxygen species,ROS)以及分泌白介素(IL)-8的量。结果:3组粘结剂的早期凋亡率、坏死率和存活率两两比较,差异有统计学意义(P<0.05),其中聚羧酸锌水门汀组早期凋亡率、坏死率最高,存活率最低;玻璃离子水门汀组早期凋亡率、坏死率最低,存活率最高。玻璃离子水门汀组的早期凋亡率与存活率与空白对照组比较差异无统计学意义(P>0.05),而3组粘结剂的坏死率均高于空白对照组(P<0.05)。PMNs释放IL-8的量,3组粘结剂均高于空白对照组,其中聚羧酸锌水门汀组最高,玻璃离子水门汀组最低,差异有统计学意义(P<0.05)。PMNs的ROS产生强度,只有玻璃离子水门汀组高于空白对照组,差异有统计学意义(P<0.05)。结论:3种粘结剂对人中性粒细胞的相关活性以及致炎性均有影响,其中玻璃离子水门汀的致炎性相对较低,抗菌性能相对较高。

Objective： To evaluate the capacity of of three cements on polymorph nuclear neutrophils. Methods： The bottom of 24-well plates were coved by glass ionomer cement, zinc polycarboxylate cement, and light-cured resin cement respectively. Polymorph nuclear neutrophils（PMNs）were isolated from blood collected from healthy volunteer donors. PMNs were co-incubated with or without cement for 4 hours. Flow cytometry was used to detect cell viability and reactive oxygen species （ROS） in PMNs. Concentration of interleukin-8 （IL-8） in supernatant fluid was detected by ELISA. Results： The early apoptosis and necrosis rate of the control group is significantly lower,while the survival rate is significantly higher than that of cement groups. For the glass ionomer group has the highest survival rate PMNs is and the lowest rates of the early apoptosis and the necrosis. For the glass iron group, the ROS level of PMNs demonstrates a significantly increase. The concentrations of IL-8 of the cement groups are significantly higher than that of the control group, and the glass ionomer cement group has the highest level, while the zinc polycarboxylate cement group has the lowest. Conclusion： The glass ionomer displays characteristics of relative excellent antibacterial activities and lower inflammatory activation.