彩色多普勒血流显像评价兔肾缺血再灌注损伤的可行性研究

The feasibility of using CDFI to evaluate rabbit renal ischemia-reperfusion injury

目的:探讨彩色多普勒血流显像(CDFI)评价肾缺血再灌注损伤发生的可行性。方法:48只大白兔随机分为假手术对照(S)组、缺血再灌注(I/R)组。应用CDFI检测各级兔肾动脉的血流动力学变化,测定兔肾组织肿瘤坏死因子α(TNF-α)的平均光密度(OD)并做相关性分析。结果:与S组比较,I/R2h组无明显血流动力学改变,I/R8h、24h组分别出现SRA、IRA的RI增大和MRA、SRA、IRA的Vmax、PI、RI增大(P<0.05)。I/R8h,24h组TNF-α的OD高于S组(P<0.05)。I/R各组MRA、SRA和IRA的Vmax,PI,RI与兔肾组织TNF-α的OD变化呈正相关(P<0.05)。结论:CDFI是一种无创、及时的评价肾缺血再灌注损伤发生的可行方法。

Objective To study the feasibility of using CDFI to evaluate rabbit renal ischemia-reperfusion injury.Methods 48 rabbits were randomly divided into sham operation control group（S） and ischemia-reperfusion injury group（I /R）.The hemodynamicparametersof rabbit renal artery and the expression of TNF-α in rabbit kidney tissue were respectively measured by color Doppler flow imaging（CDFI） and TNF-α kit.The correlationanalysis was studied between CDFI parameters andTNF-α expression.Results I /R 2 h group had no significant changes in hemodynamic parameters compared to S group.The hemodynamic parameters of I /R long time group were significantly changed such as RI of rabbits SRA and IRA.The TNF-α level of rabbits renalwas increased in I /R 8 h、 24 h group.Vmax,PI,RI of rabbits MRA,SRA and IRA had positive correlation with TNF-αlevel（P 0.05）.Conclusion CDFI is a feasible method to evaluate the incidence of renal ischemia /reperfusion injury.