普通小麦-柔软滨麦草易位系M97抗条锈基因的遗传分析和分子作图

Genetic analysis and molecular mapping of stripe rust resistance gene in wheat Leymus mollis translocation line M97

为了明确M97抗条锈性遗传规律,在苗期用7个小麦条锈菌系对M97与感病品种铭贤169的杂交后代F1、F2、F3和BC1代进行抗条锈性遗传分析,并对M97抗Sun11-4的抗条锈基因进行SSR分子标记。M97对Sun11-4和Sun11-11的抗病性均由1对显性基因控制,对CY29、CY30、CY33的抗病性由1显1隐2对基因共同控制,对CY31的抗病性由2对显性基因独立或重叠作用控制。以接种Sun11-4的F2代分离群体构建作图群体,筛选到Xwmc222、Xwmc147、Xbarc229和Xwmc339等4个与抗病基因连锁的SSR标记,其遗传距离分别为3.4、4.8、7.6和12.1 cM。将该抗病基因定位于小麦1DS染色体,且该基因不同于已知的抗条锈基因,暂命名为YrM97。用YrM97两侧遗传距离最近的2个标记Xwmc222和Xwmc147对42个黄淮麦区主栽小麦品种进行分子检测,仅有9.5%的品种具有与YrM97相同的标记位点。

In order to identify the resistance gene （s） to strip rust in M97, F1, F2, F3 and BC1 proge- nies, derived from M97 crossing with susceptible parent Mingxian169, were tested with Chinese races Sunll-4, Sunll-ll, CY29, CY30, CY31, CY32 and CY33 at the seedling stage in greenhouse. The gene conferring resistance to Sunl 1-4 in M97 was mapped by linked SSR markers. The genetic results showed that one dominant gene conferring resistance to Sunl 1-4 and Sunl 1-11 ; one dominant gene and one recessive gene conferring resistance to races CY29, CY30 and CY33 ; two dominant genes independently conferring resistance to CY31. The mapping population was constructed with F2 generation inoculated with Sunl 1-4. Four SSR markers, Xwmc222, Xwrnc147, Xbarc229 and Xwmc339, were link to the resistance gene, with genetic distance range from 3.9 to 11.2 cM. The gene was located at chromosome 1 DS and the gene maybe a new gene different from all known stripe rust resistance genes, temporarily designated as YrM97. Flanking markers of Xwmc222 and Xwrnc147 were used to test 42 wheat cuhivars from Huanghuai wheat growing region, only 9.5% cultivars have the same polymorphic bands as YrM97,which indicated the polymorphic SSR markers can be useful in wheat molecular marker select breeding.