灰霾天气细颗粒物对人支气管上皮细胞凋亡的影响

Effects of Haze Fine Particulate Matter on Apoptosis of Human Bronchial Epithelial Cells

[目的]探讨灰霾天气细颗粒物(PM2.5)对人支气管上皮细胞(human bronchial epithelial cells,16-HBE)凋亡的影响。[方法]分别以8、16、32、64、128μg/mL浓度的PM2.5作用于16-HBE 24、48、72h,检测PM2.5对16-HBE细胞存活率及凋亡率的影响。[结果]染毒24、48、72h后,64、128μg/mL组细胞存活率均明显低于对照组(P<0.05),细胞凋亡率均明显高于对照组(P<0.05);72h各浓度组细胞存活率均低于24h相应处理组(P<0.05),当染毒浓度大于8μg/mL时,72h各浓度组细胞总凋亡率与24h相比均明显增加(P<0.05)。[结论]灰霾天气细颗粒物PM2.5对16-HBE具有细胞毒性,能诱导其凋亡,且具有剂量和时间反应关系。

[Objective] To explore the effect of haze particulate matter PM2.5 on apoptosis of human bronchial epithelial cells（16-HBE）.[Methods] Groups of cultured 16-HBE cells were exposed to 8,16,32,64 and 128 μg/mL PM2.5 respectively for 24,48 and 72 h of incubation to detect the cell survival and apoptosis rates.[Results] After 24,48 and 72 h of interference,compared with the negative control group,the cell survival rates of 64 and 128 μg/mL groups decreased significantly（P 0.05）,and the cell total apoptosis rates increased significantly（P 0.05）.Compared with the corresponding 24 h groups,the cell survival rates of 72 h groups decreased significantly（P 0.05）,and the apoptosis rates increased significantly except when treated with 8 μg/mL（P 0.05）.[Conclusion] Haze particulate matter PM2.5 possess cytotoxic effect on 16-HBE to induce apoptosis in a dose-and time-dependent manner.