自主研发电化学治疗同轴电极与传统针式电极电解效应的对比研究

The electrolytic effect in electrochemotherapy:a comparative study between self-prepared coaxial electrode and conventional needle electrode

目的用自主研发同轴电极和直针式电极电解含百里香酚蓝琼脂-NaCI胶体系统模拟组织毁损,观察两者的电解效应,探讨自主研发同轴电极的应用价值。方法采用百里香酚蓝琼脂-NaCI胶体系统模拟组织。在实验中保持同轴电极和针式电极的接触面积、间距一致。随着阴极周围OH-的聚集,pH升高,阴极周围胶体会变为鲜艳的蓝色。将电流分为1.6、5.0、10.0和20 mA 4组进行实验,当电量累积到1、2、4、6、8库仑时测量阴极变色范围。结果在恒定直流电作用下,各电流组同轴电极和针式电极的阴极周围的蓝色量均随电量增加而增加,各组内同轴电极和针式电极间的作用差异无统计学意义(P>0.05)。在给予相同电量时,对各电流组阴极周围的变色体积进行组间比较,差异有统计学意义(P<0.05),即电流越小作用时间越长,变色范围越大。结论自制同轴电极与直针式电极具有相近的电解效果,可以替代直针式电极在电化学治疗中的应用。

Objective To evaluate the electrolytic effect of a self-prepared coaxial electrode and the conventional needle electrode, and to discuss the clinical application of the self-prepared coaxial electrode. Methods The agar-NaC1 colloid system, which was used as an analogue of organic tissue, was prepared for the experiment. The colloid was destructed by electrolysis by using a self-prepared coaxial electrode or a conventional needle electrode separately. The electrolytic effect of the two different electrodes was determined, and the results were compared. The anode was at the center, while the cathodes were located at the surrounding area. In the experiment, the bare surface area and the distance between the electrodes used in the experiment were the same. With the accumulation of OH- around the cathode, the pH profile rose, and meanwhile the agar-NaC1 colloid around the cathodes became bright blue in color. Based on the electric current used in the study, the experiments were divided into four groups： 1.6 mA group, 5.0 mA group, lO.0mA group and 20mA group. The color-changed size around the cathode was measured when the accumulative quantity of electric charge reached 1, 2, 4, 6 and 8 coulombs. The results were analyzed. The application of self-prepared coaxial electrode was discussed. Results （1） When the current was constant, the size of bright blue agar showed a parallel relationship with the delivered coulomb in all groups. The difference between the self-prepared coaxial electrode and the conventional needle electrode in each group was not significant （P 〉 0.05）. （2） Meanwhile, when the delivered dose was constant, the lower the current was and the longer the time was, the larger the volume of blue agar would be. The difference was statistically significant among the four groups （P 〈 0.05）. Conclusion When the current and delivered coulomb are constant, the electrolytic effect of the self-prepared coaxial electrode is quite the same as that of the conventional needle electrode. Therefore, the self-prepared coaxial electrode can replace the conventional needle electrode in electrochemotherapy.