摘要
建立了利用电场轨道阱回旋共振组合质谱仪(LTQ-Orbitrap)检测人体尿样中青霉素G及其代谢产物的分析方法。目标化合物分离鉴定选用PLRP-S聚合色谱柱,以含0.1%(体积比)甲酸溶液-乙腈为流动相,梯度洗脱分离后,选用Data-dependent MSn模式进行全扫描分析。尿样中首次检出青霉素甲酰化代谢产物(m/z 381)。青霉素新代谢物的发现对食品安全及法医毒物分析具有重要意义。
A method for confirmation of penicillin G and its metabolites in human urine using LC-LTQ-Orbitrap was developed. Urine was directly deproteinated by acetonitrile, the target compounds in urine samples were separated by PLRP-S column with the solution of 0. 1% formic acid aqueous in acetonitrile, finally analyzed with data-dependent MS＂ full scan mode. A new compound （m/z 381 ） was detected in human urine. These results could be applied to detection of drug residues in the field of food safety and analytical toxicology.
出处
《化学通报》
CAS
CSCD
北大核心
2012年第2期144-149,共6页
Chemistry
