天然恒磁场对肝癌细胞系增殖影响的研究

The effects of natural static magnetic fields on the proliferation of three human hepatocarcinoma cell lines

目的研究天然恒磁场对3个人肝癌细胞系增殖的影响。方法将3个人肝癌细胞系BEL-7402、HEPG-2及QGY-7701分别暴露于0．1T、固定600Hz频率的天然恒磁场，以及0．2T、频率分别为F1（250Hz）、F2（400Hz）、F3（500Hz）的天然恒磁场中。每天磁曝时间为30min，磁曝天数分为3d组与6d组，每组设置对照组与磁曝组。完成磁曝后以流式细胞仪及细胞增殖检测试剂盒检测细胞凋亡与增殖。以t检验对比磁曝组与对照之间结果的差别。结果仅有0．2T400Hz条件下的天然恒磁场可诱导BEL．7402细胞系凋亡，而同一条件下不能诱导其余2个人肝癌细胞系凋亡。结论同一疾病不同细胞系对同一磁场的不同反应揭示了不同细胞系之间特性的差别，其中可能的机制需进一步研究。不同细胞对同一磁场的不同反应提示在临床中应采用个体化磁场治疗方案以取得较好疗效。

Objective To observe the effects of a natural static magnetic field （NSMF） on the proliferation of human hepatocarcinoma cells. Methods Three human hepatocarcinoma cell lines （BEL-7402, HEP G-2 and QGY-7701 ） were cultured under standard conditions and then exposed to NSMFs of 0.1 Tesla at 600 Hz, 0.2 Tesla at 250 Hz, 0.2 Tesla at 400 Hz or 0.2 Tesla at 500 Hz for 30 minutes daily for 3 or 6 days. Flow eytometry and the XTT cell proliferation assay were used to check apoptosis and the proliferation of cells all groups. Results Only ex- posure to 0.2 Tesla magnetism at 400 Hz induced apoptosis of the BEL-7402 cell line. None of the NSMFs induced apoptosis in the other 2 hepatocarcinoma cell lines. Conclusions Different reactions of the three hepatocarcinoma cell lines to the same NSMF implies that each cell line has its own features, and that individualized NSMF treatment orotoeols should be adooted for better clinical outcomes.