大豆生育期相关性状QTL定位

Mapping QTLs for Growth Period Related Traits in Soybean

以野生大豆江浦野生豆-5为母本,栽培大豆南农06-17为父本杂交所得的316个F2单株及其衍生F2∶3和F2∶4家系为材料,利用JoinMap3.0软件,构建了一张包含210个标记(分子标记207个、形态标记3个),共24个连锁群的大豆分子连锁图谱,覆盖基因组长度2 205.85 cM,标记间平均距离为11.09 cM。利用混合线性模型复合区间作图方法,对2007年F2单株、2008年F2∶3家系及2009年F2∶4家系的全生育期、营养生长期、生殖生长期和生育期结构4个生育期相关性状进行联合世代QTL分析,共检测到15个加性显性QTL和9对上位性QTL;存在QTL共位性(同一标记区间存在不同性状的QTL)以及QTL互作网络(一个QTL可以与多个QTL互作)的现象;贡献率最大的3个QTL为qVP-H-1、qWGP-H-1和qRV-H-1,加性效应解释的遗传变异分别为21.31%、13.14%和9.37%,qWGP-H-1和qVP-H-1的增效等位基因来源于江浦野生豆-5,qRV-H-1的增效等位基因来源于南农06-17。研究结果为生育期性状的分子标记辅助选择、野生大豆优异基因的挖掘及栽培大豆遗传基础的拓宽提供了依据。

One F2 population（316 plants）derived from a cross between Jiangpu wild soybean-5 and cultivar soybean line Nannong 06-17 was used to construct a genetic linkage map by JoinMap 3.0.In total,210 markers（207 molecular markers and 3 morphological markers）were mapped.The constructed linkage map covered 2 205.85 cM and consisted of 24 linkage groups（LGs）,with an average distance between markers of 11.09 cM.Four growth period related traits including whole growth period（WGP）,vegetative period（VP）,reproductive period（RP）and growth period structure trait（RV）of 2007 F2,2008 F2∶3 and 2009 F2∶4 were mapped by mixed-model-based composite interval mapping（MCIM）.We detected 15 additive-dominant QTLs and 9 pairs of epistatic QTLs.QTL clusters（QTLs of different traits in the same marker）and QTLs interaction networks（the interaction among QTLs）were found.Three major QTLs were qVP-H-1,qWGP-H-1 and qRV-H-1.Additive effect of qVP-H-1,qWGP-H-1 and qRV-H-1 accounted for 21.31%,13.14% and 9.37% genetic variation respectively.These results will provide a basis for molecular marker-assisted selection,elite genes-discovering and soybean genetic basis-broadening.