大豆体细胞胚的诱导及对草甘膦耐性的研究

Induction of Soybean Somatic Embryos and Their Tolerance to Glyphosate

以大豆未成熟子叶为外植体,研究合丰25、吉林35、吉育91对未成熟子叶体细胞胚诱导率的影响,并探讨3种基因型大豆未成熟子叶对草甘膦的耐性。结果表明:3种基因型的愈伤组织诱导率均为100%,体细胞胚诱导率为53.95%～72.12%,平均胚数吉育91高于其它2种基因型。3种基因型的体细胞胚诱导率在草甘膦浓度间存在差异,在大豆以未成熟子叶为受体转基因时,诱导体细胞胚胎发生的草甘膦筛选浓度为2.5～5.0 mg.L-1。

Immature cotyledons of Hefeng 25,Jilin 35 and Jiyu 91 were used as explants to study the effects of genotypes on the somatic embryogenesis of soybean.Tolerance of soybean immature cotyledons to glyphosate in three genotypes was also investigated.Induction rate of calli in three genotypes raised to 100%.The induction rate of somatic embryogenesis ranged from 53.95% to 72.12% among three genotypes and the average number of somatic embryo in Jiyu 91 was higher than Hefeng 25 and Jilin 35.The induction rate of somatic embryogenesis varied with glyphosate concentrations.The results suggest that somatic embryogenesis should be selected by using 2.5-5.0 mg·L-1 of glyphosate for transformation with immature cotyledons as explants in soybean.