溶胶-凝胶生物活性玻璃对人牙髓细胞作用的研究

Effects of ionic-dissolution of sol-gel bioactive glasses on human dental pulp cells

目的:研究溶胶-凝胶生物活性玻璃对人牙髓细胞增殖与碱性磷酸酶(alkaline phosphatase,ALP)活性的作用。方法:取人无龋前磨牙或第三磨牙的牙髓进行牙髓细胞原代培养,传至第4代作为实验用细胞。实验用生物活性玻璃包括溶胶-凝胶法制备的58S、纳米58S(Nano-58S)和传统熔融法制备的45S5。用含有1 g/L(mg/mL)的58S、Nano-58S和45S5浸提液的达尔伯克必需基本培养基(Dulbecco’s mimimum essential medium,DMEM)培养人牙髓细胞,对照组为不含生物活性玻璃的DMEM培养组。采用甲基噻唑基四唑溶液[(4,5-dimethylthiazol-2-yl)2,5-diphenyl tetrazolium bromide,MTT]法于第1、3、5、7天测定细胞增殖,第14天检测细胞的ALP活性。结果:Nano-58S组的细胞增殖显著高于对照组,Nano-58S组和58S组的细胞ALP活性显著高于对照组(P<0.01);45S5组的细胞增殖(P<0.01)和ALP活性(P<0.05)显著低于对照组。结论:Nano-58S和58S溶胶-凝胶生物活性玻璃能够促进人牙髓细胞增殖和ALP活性,对人牙髓细胞具有良好的生物活性作用。

Objective： To evaluate the effects of ionic-dissolution of sol-gel bioactive glasses（BG） on proliferation and alkaline phosphatase（ALP） activity of human dental pulp cells（HDPCs）.Methods： The primary HDPCs were isolated from intact premolar and third molars and were cultured in DMEM.Then the 4 th generation of HDPCs were cultured in DMEM,which contained 1 g/L of one of 58S,Nano-58S,and 45S5 ionic-dissolution products.Meanwhile HDPCs were cultured in DMEM without BG as control group.Proliferation of the cells was evaluated by MTT assay on days 1,3,5 and 7.ALP activity was measured on day 14 using ALP Assay Kit.Results： Compared with the control group,HDPCs＇ proliferation in the Nano-58S group increased significantly.HDPCs cultured in Nano-58S and the 58S groups showed higher ALP activity（P0.01）;but in 45S5 group showed an inhibition on the HDPCs＇ proli-feration（P0.01） and lower ALP activity（P0.05）.Conclusion： Present work has shown that Nano-58S and 58S sol-gel BG can promote the proliferation and ALP activity of HDPCs.