人脂肪基质细胞在骨组织工程学中的应用

Application of human adipose-derived stromal cells in bone tissue engineering

口腔颌面部及全身骨组织缺损是临床医生经常要面对的困境。造成骨缺损的原因有很多,包括先天发育异常、炎症、肿瘤、外伤等,然而治疗骨缺损的方法却十分有限,以往主要应用自体骨移植或人工骨替代材料,但是,自体骨移植取材受限并且会增加手术创伤,人工材料生物相容性差并且成骨能力有限,

SUMMARY Human adipose-derived stromal cells（hASCs） can be obtained from adipose tissues that offer an abundant and easily accessible pool of stem cells.Thus,hASCs have become a highly attractive source of seed cells in bone tissue engineering and have promising prospects in bone regeneration.Since 2002,our research group has performed a series of experiments on hASCs and its application in bone tissue engineering,including： to substitute dexamethasone by 1,25（OH） 2vitamin D3to induce osteogenic differentiation of hASCs;to explore the effect of epigenetic regulation and to inflammation on the osteogenic differentiation of hASCs;to construct a novel and simple tissue engineered bone system by hASCs and human platelet-rich plasma（hPRP） and to investigate the bone formation capability of this tissue engineered bone and the stimulatory effect of simvastatin.Our results suggested that 1,25（OH） 2vitamin D3 could replace dexamethasone to induce the osteogenic differentiation of hASCs;retinoblastoma binding protein 2（RBP2）,as one of histone demethylases,could regulate the osteogenic differentiation of hASCs epigenetically while tumor necrosis factor α（TNFα）,as a inflammatory factor,could also influence the osteogenic differentiation of hASCs.Moreover,we found that in vivo bone formation could be detected by our novel tissue engineered bone composed with hASCs and hPRP;simvastatin could enhance the bone formation capability of this tissue-engineered structure.