摘要
目的:确定大黄、黄芩与黄柏药对的最佳配伍提取工艺。方法:将各药味进行配伍组合,以大黄素、大黄酚、黄芩苷、盐酸小檗碱提取率为评价指标,采用HPLC测定,以正交试验考察乙醇浓度、乙醇用量和提取次数等因素的影响,优化最佳提取工艺。结果:大黄与黄芩合提,黄柏单提指标成分提取率较高,得到合提最佳工艺为12倍量60%乙醇溶液,回流提取3次,每次1 h;单提最佳工艺为10倍量60%乙醇溶液,回流提取3次,每次1 h。结论:所确定的配伍提取工艺合理、稳定可行。
Objective:To determine the optimum compatibility extraction technology of Rhei Radix et Rhizoma,Scutellariae Radix and Phellodendri Chinensis Cortex.Method:The drugs were combined respectively firstly,then use HPLC to determinate extraction rates of evaluating indicators including emodin,chrysophano,baicalin and berberine hydrochloride.Orthogonal experiment was used to detect the effect on concentration and volume of ethanol,times of extraction.Result:The extraction rates of evaluating indicators were relatively high in combined extraction of Rhei Radix et Rhizoma with Scutellariae Radix and single extraction of Phellodendri Chinensis Cortex.The optimized combined extraction were 12 times more than the volume of 60% ethanol solution,by boiling for 3 times,each for 1.0 hour,and that of single extraction were 10 times more than the volume of 60% ethanol solution,by boiling for 3 times,each for 1.0 hour.Conclusion:The optimized compatibility extraction technology is reasonable,stable and practical.
出处
《中国中药杂志》
CAS
CSCD
北大核心
2012年第4期461-465,共5页
China Journal of Chinese Materia Medica
基金
浙江省教育厅高校科研基金项目(20060717)
浙江省科技厅重点项目(2007C23020)
浙江省中药现代化专项资金项目
浙江省中医药科研基金项目(2006C038)
关键词
散瘀方
大黄
黄芩
黄柏
提取工艺
Sanyu prescription
Rhei Radix et Rhizoma
Scutellariae Radix
Phellodendri Chinensis Cortex
extraction technology