UPLC-MS/MS同时测定大鼠血浆中异荭草素,野黄芩苷和木犀草苷及其药代动力学研究

Simultaneous determination of isoorientin,scutellarin and cynaroside in rat plasma and pharmacokinetics by UPLC-MS/MS

目的:建立利用UPLC-MS/MS同时测定大鼠血浆中异荭草素、野黄芩苷和木犀草苷等3种黄酮的分析方法,并研究大鼠静脉注射3个剂量的注射用复方荭草后的药代动力学特征。方法:血浆样品采用酸化后甲醇沉淀蛋白,采用WatersAcquity BEH C18色谱柱,流动相为0.1%甲酸乙腈-0.1%甲酸水梯度洗脱,采用电喷雾电离源(ESI),扫描方式为多反应离子监测(MRM)。结果:3种黄酮成分在大鼠血浆中线性关系良好,提取回收率78.56%～101.91%,日内、日间精密度和准确度良好,各物质在大鼠体内的平均滞留时间均较短(<22 min)。结论:该方法特异、快速、准确和灵敏,可用于注射用复方荭草在大鼠体内的药动学研究。

Objective:To establish a UPLC-MS/MS analysical method for simultaneous determination of concentrations of isoorientin,scutellarin and cynaroside in rat plasma and to study their pharmacokinetic characteristics after intravenous injection of 3 doses of Fufang Hongcao in rats.Method:Acidified plasma samples were precipitated for protein with methanol.Waters Acquity BEH C18 column was adopted for spectrum,with mobile phase as 0.1% formic acid acetonitrile-0.1% formic acid-water gradient elution.Detection was carried out by the multiple reaction monitoring(MRM) positive ion mode with ESI ionization source.Result:Three flavonoids show a good linear relationship,with the extraction recovery ranging between 78.56% and 101.91% and a high intra-and inter-day precisions and accuracy.The MRT of the three flavonoids were all lower than 22 min in rats.Conclusion:The above mentioned method is so specific,rapid,sensitive that it is suitable for pharmacokinetic studies of Fufang Hongcao injection in rats.