大肠癌细胞增殖活性与淋巴结转移规律的研究

采用流式细胞术(FCM)测定了30例大肠癌患者新鲜癌组织标本的DNA指数(DI)、S期细胞比率(SPF)及增殖指数(PI),同时对30例大肠癌患者的淋巴结转移状况按转移个数和转移距离进行分站。结果显示,大肠癌异倍体淋巴结转移率较二倍体淋巴结转移率明显升高(P<0.05)。大肠癌淋巴结转移阳性组较阴性组SPF、PI明显升高,淋巴结多数转移组较少数转移组、少数转移组较无转移组SPF、PI明显升高,N2站转移组较N1站转移组、N1站转移组较N0站转移组SPF和PI升高。认为随着增殖活性的升高,肿瘤发生转移的范围更广、距离更远,提示肿瘤恶性程度较高,预后不良。

The DNA index(DI),s phase fraction(SPF)and proliferative index(PT)were determinated in 30 fresh tumor samples by flow cytometry(FCM) The tumors were classified by the number or the extent of lymph node metastasis The results showed that in aneuploidy tumors the incidences of lymph node metastasis were higher than that in diploid tumors(P<0 05) The aneuploid tumors had higher SPF and PI values than the diploid tumors The SPF and PI values of tumors with lymph node metastasis were higher than those without lymph node metastasis The SPF and PI values correlated with the number of lymph node metastasis The SPF and PI values were significantly higher in metastasis to 4 lymph node or more than to 1 to 3 lymph node and to 1 to 3 lymph nodes than to 0 lymph nodes Values were also found higher in metastasis to N 2 than to N 1 and to N 1 than to N 0 It suggests that tumors of higher proliferative activity might be more malignant and aggressive Proliferative activity might be a useful parameter in evaluating prognosis