多药耐药相关蛋白在胃癌中的表达及其临床意义

采用免疫组化法检测36例胃癌患者癌组织中的多药耐药相关蛋白(MRP)表达情况,其中14例同时应用流式细胞术(FCM)检测MRP表达率。结果36例胃癌组织中29例MRP表达阳性,阳性率为80.6%,MRP表达与肿瘤大小、组织学类型、淋巴结转移、浸润深度及TNM分期无关。FCM检测结果显示,MRP的平均表达率为39.1±13.4%,明显高于正常对照(11.9±2.5%,P<0.01)和阴性对照(1.9±0.1%,P<0.01)。认为胃癌组织存在较高的原发耐药,MRP测定有助于选择化疗药物。

The multidrug resistance associated protein(MRP)in cancer tissue of 36 patients with gastric cancer was measured by immunohistochemical staining,and the MRP of 14 of them were also detected by flow cytometry(FCM) The results showed that the MRP expressed in 29 of 36 specimens There was no correlation between the MRP expression and tumor size,histologic types,lymph nodal metastasis,serosal invasion,and TNM stage The mean MRP positivity rate detected by FCM is 39 1%±13 4%,much higher than these in normal(11 9%±2 5%,P<0 01)and negative(1 9%±0 1%,P<0 01)controls This indicates that the gastric cancer exists highly intrinsic resistance,and the analysis of MRP can help to choose the more effective chemotherapy druds