摘要
目的建立反相高效液相色谱法测定六味地黄丸(熟地黄、山茱萸、牡丹皮等)中芍药苷、丹皮酚和乌苏酸的方法。方法测定芍药苷和丹皮酚用Thermo ODS C18色谱柱,流动相为甲醇-0.05%磷酸梯度洗脱,体积流量1.0 mL/min,最大吸收波长下检测(芍药苷在230 nm,丹皮酚在274 nm);测定乌苏酸用SunFire C18色谱柱,流动相为乙腈-甲醇-磷酸盐缓冲液(pH6.5)(70∶15∶15),体积流量1.0 mL/min,检测波长210 nm。结果芍药苷在0.194～2.425μg,丹皮酚在0.159～1.985μg,乌苏酸在0.376～3.760μg范围内,均呈良好线性关系,相关系数分别为0.999 5、0.999 9、0.999 0,平均回收率为芍药苷99.73%(RSD为2.29%),丹皮酚101.15%(RSD为1.44%),乌苏酸96.72%(RSD为1.86%)。结论该方法简便、准确、重复性好,可用于六味地黄丸的质量控制。
AIM To establish RP-HPLC method for determining paeoniflorin,paeonol and ursolic acid in Liuwei Dihuang Pill（Rehmanniae Radix Praeparata,Corni Fructus,Moutan Cortex,etc.）.METHODS Thermo ODS C18 column was used to determine paeoniflorin and paeonol,with the mobile phase consisting of methanol-0.1% phosphoric acid in gradient elution,at the flow rate of 1.0 mL/min.The UV detection wavelength was set at 230 nm for paeoniflorin,and 274 nm for paeonol.SunFire C18 column was used to determine ursolic acid,with the mobile phase consisting of acetonitrile-methanol-phosphate buffer solution（pH 6.5）（70∶ 15∶ 15）,at the flow rate of 1.0 mL/ min.The UV detection wavelength was set at 210 nm.RESULTS The linear ranges of paeoniflorin,paeonol and ursolic acid were 0.194-2.425 μg（r=0.999 5）,0.159-1.985 μg（r=0.999 9）,and 0.376-3.760 μg（r=0.999 0）,respectively.The average recoveries of paeoniflorin,paeonol and ursolic acid were 99.73%（RSD was 2.29%）,101.15%（RSD was 1.44%）,and 96.72%（RSD was 1.86%）,respectively.CONCLUSION The method is sample,accurate and reproducible.It can be used for the quality control of Liuwei Dihuang Pill.
出处
《中成药》
CAS
CSCD
北大核心
2012年第2期277-282,共6页
Chinese Traditional Patent Medicine
