氯离子通道蛋白及β-肌动蛋白基因在人胃癌组织中的表达

Expression of Chloride Ion Channel Protein andβ-actin Genes in Human Gastric Cancer and Its Relationship with Clinicopathologic Features

目的:研究Hp(Helicobacter pylori,Hp)感染在胃癌发生发展中的作用。方法:运用实时定量PCR法检测氯离子通道蛋白基因(Chloride ion channel protein,CICP)及β-肌动蛋白基因在50例人胃癌组织的表达,并分析其表达与临床病理特征和胃癌组织中Hp感染状态的关系。结果:两个基因在人胃癌组织及淋巴结组织的表达显著高于癌旁组织(P<0.05)。氯离子通道蛋白基因的表达与胃癌患者pTNM分期及患者生存期相关(P<0.05),而β-肌动蛋白基因的表达与pTNM分期及淋巴结转移相关(P<0.05);人胃癌组织中Hp的感染与氯离子通道蛋白及β-肌动蛋白基因的表达上调相关(P<0.05)。结论:Hp的感染可以上调胃癌组织中氯离子通道蛋白及β-肌动蛋白基因的表达,检测这两个基因的表达对预测胃癌患者的预后有一定作用。

Objective： To explore the role of Hp infection in the pathogenesis and progression of gastric cancer. Methods： Expression of chloride ion channel protein （ CICP ） and β-actin genes was detected in gastric cancer tissue, lymph nodes, and paraneoplastic tissue from 50 gastric cancer patients were detected using real-time fluorescence quantitative polymerase chain reaction. The relationships among the expression of these genes and the clinicopathologic features in the patients, as well as gastric cancer and Hp infection, were analyzed. Results： The expression of CICP and β-actin genes in gastric cancer and lymph node tissue significantly increased, compared with the paraneoplastic tissue （ P 〈 0.05 ）. CICP and β-actin gene expression showed a positive correlation （ P 〈 0.05 ） with the pTNM stage, survival time, and lymph node metastasis. The over-expression of both genes was related to the Hp infection in gastric cancer tissue （ P 〈 0.05 ）. Conclusion： Hp infection significantly up-regulates the expressions of CICP and β-actin genes in patients with gastric cancer, and the detection of both genes may be helpful in the prediction of the prognosis of these patients.