BHLHB2参与人胰腺癌细胞的迁徙、凋亡及EMT

BHLHB2 participates in cell migration,cell apoptosis and EMT in human pancreatic cancer

目的进一步研究前期实验结果证明的人胰腺癌新颖的独立预后因子BHLHB2在人胰腺癌细胞中的相关分子生物学功能。方法应用siRNA干扰技术,在人胰腺癌细胞株SU86.86中使BHLHB2表达沉寂,应用迁徙实验研究BHLHB2与细胞迁徙的关系;应用更生霉素(ACT-D)诱导细胞凋亡,研究BHLHB2与细胞凋亡的关系;应用Western blot方法研究BHLHB2与EMT的相关性。结果人胰腺癌细胞BHLHB2表达沉寂后,细胞的迁徙明显增快。应用ACT-D进行凋亡诱导,BHLHB2表达沉寂后与对照组相比,发生凋亡的细胞数目显著增加(10μg/L,P<0.05;100μg/L,P<0.05)。Western blot结果表明,BHLHB2表达沉寂后可显著降低E-cadherin的表达(P<0.01)。结论 BHLHB2与人胰腺癌细胞的迁徙、细胞凋亡及EMT密切相关。

Objective To further explore BHLHB2 related molecular biology functions,which was proved to be a novel and independent prognostic marker in pancreatic ductal adenocarcinoma.Methods Knock-down of BHLHB2 in pancreatic cancer cell line-SU86.86 was achieved by siRNA treatment.Migration essay was performed to research the relationship between BHLHB2 and cell migration.The proliferation assay induced by ACT-D to explore BHLHB2 roles in cell apoptosis.Relationship between BHLHB2 and EMT was analyzed using Western blot.Results BHLHB2 silencing with RNAi significantly promote cell migrate,and induced cell apoptosis by Actinomycin D（ACT-D）（10 μg/L,increased 1.32 fold,P0.05;100 μg/L,increased 1.41 fold,P0.05）.By Western blot,BHLHB2 silencing significantly deduced E-cadherin expression（decreased 1.28 fold,P0.01）,which implicated that BHLHB2 was correlated with epithelial mesenchymal transform（EMT）.Conclusions BHLHB2 closely associated with cell migration,cell apoptosis and EMT in human pancreatic cancer.