摘要
在真核细胞中,核小体是组成染色质的基本结构单位,是由DNA紧密缠绕在组蛋白八聚体上所形成的一个复合体结构。而DNA与组蛋白的结合并不是固定不变的,没有核小体结合的DNA区域易于各种调节蛋白的接近与结合。因此人们怀疑核小体的定位与基因的转录调节之间存在某种内在联系。对现行的核小体定位的检测方法进行了归类,并对其优缺点进行了分析整理。对更深入的探索核小体定位检测方法的应用有一定意义。
In eukaryotes,Nucleosomes are the basic unit of DNA packaging,consisting of a segment of DNA wound around a histone protein core.Although the nucleosome is a very stable protein-DNA complex,it is not static and those DNA which are nucleosome free seems more easily to binding proteins which involved in transcriptional regulation.And this would suggest that there may have some interactions between nucleosome positioning and transcriptional regulation.In this article we will discuss the advantages and disadvantages between different methos of nucleosome positioning.And Studying the different methods of nucleosome positioning may contributes to further understanding the range of its application.
出处
《江西科学》
2012年第1期50-52,82,共4页
Jiangxi Science
关键词
核小体
染色质免疫共沉淀
微球菌核酸酶
基因芯片
高通量测序
Nucleosome
Chromatin immunoprecipitation
Micrococcal nuclease
Gene chip
High-throughput sequencing
