诱导方式及基因型对小黑麦单倍体形成的影响

EFFECT OF INDUCTION METHODS AND GENOTYPES OF TRITICALE AND MAIZE ON TRITICALE HAPLOID PRODUCTION

为探索小黑麦单倍体胚诱导方式及小黑麦基因型与玉米类型对小黑麦单倍体胚产生的影响,本文采用4种不同诱导方式、3个小黑麦基因型与3个不同类型的玉米分别对其在小黑麦单倍体胚诱导中的效果进行了研究。结果表明:以玉米花粉授粉后再辅以100mg/L 2,4-D+30ml/L二甲基亚砜对小黑麦单倍体胚的诱导效果最好,其单倍体诱导率为6.65%。不同小黑麦基因型在玉米花粉诱导小黑麦单倍体中的诱导效果是不同的,本研究中以基因型H09-4为最高;不同玉米类型远缘杂交的得胚率总趋势表现为:爆裂玉米ZPA 82/9(4.71%)>甜玉米新自31162(2.94%)>普通玉米103A(1.76%)。可见,除诱导方式外,小黑麦基因型及玉米类型对小黑麦单倍体胚的诱导效果具有重要影响。

To study the effect of induction methods and genotypes of triticale and maize genotypes on triticale haploid induction, four different induction methods, 3 triticale genotypes and 3 types of maize were used. The results indicated that the triticale pollinated by maize pollen and then treated with 100mg/L 2, 4-D combined with 30ml/L DMSO was the best induction method with triticale haploid rate of 6.65%. Different triticale genotypes had different haploid rates, and H094 was the best one with highest haploid embryos induction rates. Different maize types could induce different triticale haploid frequencies, and popcorn was better than sweet corn and normal corn for its higher frequency of triticale haploid embryo formation.