盐胁迫下AM菌侵染的棉花幼苗根系蛋白质组分析

PROTEOME ANALYSIS OF ROOTS OF COTTON SEEDLING ON AM INFECTING BY SALT STRESS

研究了盐胁迫对棉花幼苗生长的抑制作用和AM真菌侵染根系对盐胁迫的缓解作用。分别以水(对照),0.5%NaCl(盐胁迫组)和AM组(盐胁迫组中加入AM接种剂,浓度为12g/L)培养棉花幼苗30d,取根部进行外观比较,并提取各组根系中的蛋白质,利用双向电泳技术分析棉花幼苗根系蛋白质组的变化。结果表明,盐胁迫组的幼苗根系主根稍细,侧根很少,而对照和AM组主根饱满且侧根丰富,说明AM真菌能抑制盐对幼苗根部的胁迫作用。通过扫描分析3组胶图,发现有4个蛋白质斑点表现出显著的变化,盐胁迫组中均表现为表达量下降,在AM真菌与盐共处理时,这4个蛋白质点的表达均有不同程度的恢复;经鉴定分析,其中2个蛋白质斑点(S1,S2)分别被鉴定为葡萄糖磷酸变位酶与异黄酮还原酶类;同时在AM组还出现3种对照中没有的蛋白质(S5、S6、S7),可能与AM提高作物耐盐性途径相关。

The relieving effect of AM on cotton seedling growth under salt stress was studied. The seedling were cultured for 30d under three treatments of control （H20） , salt stress group（0.5% NaC1） , AM group（ （0.5% NaC1 and 12g/L AM） . Roots appearance was compared and proteins was extracted from roots. Changes of roots protein was analyzed by two- dimensional gel electrophoresis. Stronger main roots and more lateral roots were observed under AM treatment , which showed AM could inhibit the effect of salt stress on cotton seedling. By scanning gel picture of three treatments, significant changes of four protein spots were found. Less expression of these four proteins measured in salt stress group, but the expression recovered by AM treatment. It was identified that one protein （S1）was phosphoglucomutase, and the other protein （S2） was isoflavone reductase - like. Three new proteins were found only in AM group, which may relate to the increasing salt tolerance of crop.