肢体缺血预适应增强脂联素对心功能的保护作用

Limb ischemic preconditioning improved the protective effect of Adiponectin on Myocardial function

目的：研究肢体缺血预适应对缺血大鼠心肌是否具有保护作用。方法：健康雄性SD大鼠,体重220~280 g,随机分为3组（n=10）,假手术组（sham组）只穿线,左冠状动脉前降支不予处理;心肌缺血2 h组（MI2h组）：心脏冠状动脉左前降支实施缺血2 h;肢体缺血预适应组（LIPC组）：肢体缺血5 min,再灌注5 min,连续预适应3天,第4天实施缺血2 h。RM6240记录实验各组心功能指标,Elisa检测血清脂联素。结果：MI2h组大鼠血清中脂联素的含量、LVSP和±dp/dtmax与sham组比较均降低明显（P〈0.05）;LIPC组大鼠血清中脂联素的含量、LVSP和±dp/dtmax较MI2h组升高（P〈0.05）;与sham组相比,MI2h组LVEDP明显升高（P〈0.05）,与MI2h相比LIPC组LVEDP数值降低显著（P〈0.05）;血清脂联素水平与心收缩功能指标呈显著正相关。结论：脂联素与肢体缺血预适应作为正性调节因素,共同在心肌缺血损伤心肌病变的发生、发展中起作用。

Objective：To study the protection of limb ischemic preconditioning against myocardial ischemia（MI） injury in rats.Methods：SD rats（male,weight,220~280g）.Rats were divided randomly into 3 groups（n=10）.Group S（group in sham operated） were processed identically except the left coronary artery was not ligated;Group MI 2h（group in ischemia 2h） were subjected to occlusion of the left coronary artery anterior descending（LAD） followed by occlusion for 2h;Group LIPC（group in limb ischemic preconditioning） were subjected to ischemia on the left hind limb for 5 min in turn for 3 days.LAD was performed ischemia for 2h at the forth day.The function index of cardiac was recorded by RM6240.The titer of adiponectin was observed by Elisa.Results： Compared with Group S,the ADP level of blood plasma,LVSP and±dp/dtmax of Group MI2h decreased obviously（P0.05）;Compared with Group MI2h,the ADP level of blood plasma,LVSP and ±dp/dtmax of Group LIPC increased significantly（P0.05）;Compared with Group S,LVEDP of Group MI2h increased obviously（P0.05）;Compared with Group MI2h,LVEDP of Group LIPC decreased significantly（P0.05）.The level of serum adiponectin was correlated significant positively with heart systolic function parameters.Conclusions： As a positive regulated-factor,ADP and limb ischemic preconditioning worked on myocardial lesions of myocardial ischemia injury.