P16基因蛋白在原发性肝癌及癌旁组织中的表达及其临床意义

Expression and significance of P16 protein in hepatocellular carcinoma and tissues surrounding carcinoma

目的 探讨抑癌基因p1 6在肝癌及癌旁组织中的表达及临床意义。方法 应用免疫组织化学方法对 5 0例肝细胞性肝癌 (HCC)及癌旁肝硬化组织 (44例 )手术切除石蜡包埋标本进行P1 6基因蛋白表达的测定 ,并结合临床病理指标 (Child分级、肿瘤大小、Edmondson分级 ,转移及生存期 )进行分析。结果 p1 6基因在HCC中表达的阳性率为 48% (2 4 5 0 ) ,癌旁 91 % (40 4 4)。p1 6基因表达与HCC的分化程度密切相关 (r =- 0 34 4,P =0 0 1 4 ) ,在分化Ⅰ Ⅱ级中p1 6阳性率为 5 9% (1 9 32 )明显高于Ⅲ Ⅳ级的 2 7 8% (5 1 8)。结论 p1 6基因在HCC的发生及分化中起重要的调控作用 ,且p1 6的失活可能发生在HCC的早期。

Objective To investigate the expression of carcinoma suppressor gene p16 in primary hepatocellular carcinoma (HCC) and its clinical significance. Methods Samples from 50 cases of HCC and 44 cases of liver tissue surrounding carcinoma were studied by immunohistochemical technique. Results The positive rate of p16 expression was 48% in carcinoma, 91% in tissue surrounding cancer. The expression of p16 was significantly correlated with degree of differentiation (P=0.014): the positive rate in Edmondson′s Ⅰ Ⅱ grade (59%) was higher than in Ⅲ Ⅳ grade (27.8%). Conclusions The deletion and mutation of p16 gene exist in HCC, and might be the early event of HCC. p16 Gene expression may play an important role in differentiation of HCC.