门静脉营养对大鼠肝切除后肝脏再生的影响

Theinfluenceofportalnutritiononliverregenerationafterhepatectomyisrats

目的 探讨门静脉营养对肝切除后肝脏再生的影响。方法 将Wistar大鼠 40只随机分为 3组 :Ⅰ组不手术 ,自由摄食 ;Ⅱ组 6 5 %肝切除后用中心静脉营养治疗 ;Ⅲ组术后门静脉营养治疗。实验第 3、6日观察肝脏再生反应。结果 Ⅲ组肝重 体重、肝脏再生率、肝细胞有丝分裂率和DNA合成率与Ⅱ组比差异有显著意义 (P <0 0 5 ) ;Ⅱ、Ⅲ组术后胰岛素水平与Ⅰ组差异无显著意义 ,而胰高糖素水平明显升高 (P<0 0 5 ) ,且Ⅲ组明显高于Ⅱ组 (P <0 0 5 ) ,致使Ⅲ组胰岛素 胰高糖素比值明显低于Ⅱ组 (P <0 0 5 ) ,肝脏脂肪变性明显减轻。结论 大鼠肝切除后门静脉营养能降低血胰岛素 胰高糖素比值 ,减轻肝脏脂肪变性 ,促进肝脏再生。

Objective Thisstudywasdesignedtoinvestigatetheeffectofportalnutritiononliverregener ationafter 6 5 %hepatectomy.Methods Theratswereallocatedrandomlytooneofthreegroups(G) :ratsinGⅠ receivedstandardfodderadlibitumandweredevoidofhepatectomy ;inGⅡreceivedparenteralnutritionviacen tralveinafter 6 5 %liverresection ;andinGⅢreceivedthesameparenteralnutritionviaportalveinafterhepatec tomy .Theresponseofliverregenerationwasinvestigatedonthethirdandsixthdayafterliverresection .Results InGⅢ ,thehepaticmassindex ,rateofliverregeneration ,DNAsynthesis,andmitoticindexwereincreasedsig nificantlythanthoseinGⅡ (P <0 .0 5 ) .ThelevelofseruminsulininGⅡandGⅢwasnotincreasedobvious ly ,butthevalueofglucagonwasincreasedsignificantlythanthatinGⅠ (P <0 .0 5 ) ,alsotheglucagoninGⅢ wasincreasedsignificantlythanthatinGⅡ (P <0 .0 5 )andthesteatosisofliverwasreducedobviously .Conclu sions Portalnutritiondecreasedobviouslytheratioofinsulintoglucagonandreducedsteatosiscouldenhanceliver regenerationafterhepatectomy .