儿童T-淋巴细胞DNA、蛋白质合成及低剂量辐射效应的研究

Study on synthesis of DNA and protein of T-lymphocyte in children and effect of low dose radiation.

目的 观察低剂量辐射对儿童T -淋巴细胞DNA、蛋白质合成能力的影响。方法 通过体外细胞培养 ,用14 C -胸腺嘧啶脱氧核苷 (14 C -TdR)、3H -酪氨酸 (3H -Tyr)掺入法观察。 结果 在 5及 10cGy低剂量辐射下14 C -TdR、3H -Tyr掺入均有显著增加。婴幼儿组在 5cGy辐射下增加最为显著。学龄前及学龄儿童则在 10cGy辐射下增加最为显著。营养不良患儿与婴幼儿组相比较在自然状态下14 C -TdR掺入明显下降 ,不同低剂量辐射作用趋势相似 ,但强度偏低。结论 适宜的低剂量辐射可刺激儿童T -淋巴细胞DNA、蛋白质合成。

Objective To observe the effect of low dose radiation on synthesis of DNA and protein of T-lymphocyte in children.Methods To use incorporation of 14 C-TdR and 3 H-Tyr by cell culture in vitro.Results The incorporation rate of 14 C-TdR and 3 H-Tyr increased significantly under 5 and 10cGy low dose radiation.In infant group,and pre-school and school age children group,it had the most significant increase under 5cGy and 10cGy respectively .In natural state,the incorporation rate of 14 C-TdR in malnutrition children group was significantly lower than that in infant group.The tendency of effect of different low dose radiation was similar,but the intensity was relative low.Conclusion A suitable low dose radiation can stimulate synthesis of DNA and protein of T-lymphocyte in children.The stimulating effect in malnutrition children is lower than that in healthy children.