外源性孕激素补充对胚胎着床期的免疫作用

Immunomodulatory effect of exogenous progesterone supplement during implantation stage

目的 探讨外源性孕激素补充对胚胎着床期免疫的影响及其影响途径.方法 孕4、5、6天收集地屈孕酮和黄体酮干预孕鼠的子宫及血清;孕6天收集同基因交配孕鼠和异基因交配孕鼠子宫及血清,用免疫组化法检测子宫内膜孕酮诱导的封闭因子、雌孕激素受体表达,用放射免疫法检测血清雌孕激素含量.结果 异基因组较同基因组子宫内膜孕酮诱导的封闭因子、雌孕激素受体表达及血清雌孕激素含量均显著升高（t值分别为7.034,2.175,2.732,2.592,2.937,均P〈0.05）.外源性孕激素补充的孕鼠其子宫内膜孕酮诱导的封闭因子表达明显增高（t=2.090,P〈0.05）,且地屈孕酮组较黄体酮组更高（t=3.419,P〈0.01）.结论 孕酮诱导封闭因子的表达需要异基因胚胎抗原的刺激;地屈孕酮的免疫调节作用强于黄体酮.

Objective To study the immunomodulatory effect of exogenous progesterone supplement during implantation stage and the influence approach. Methods Blood and uterus samples of mice interfered with dydrogesterone and progesterone were collected on pregnant day 4, 5, and 6, and the blood and uterus samples of syngeneic group and allogeneic group were collected on pregnant day 6. Progesterone induced blocking factor （PIBF）, estrogen receptor-α （ER-α） and progesterone receptor （PR） were measured with immuohistochemistry. Serum estrogen and progesterone level were detected by radioimmunoassay. Results The expression of PIBF, ER-α and PR and the serum level of estrogen and progesterone in allogeneie pregnant mice group were higher than those in syngeneic pregnant mice group （ t value was 7. 034, 2. 175, 2. 732, 2. 592 and 2. 937 respectively, all P 〈 0.05 ）. The expression of PIBF in mice with exogenous progesterone supplement elevated significantly （ t = 2. 090 ,P 〈 0.05 ）, and it was higher in dydrogesterone group than in progesterone group （ t = 3. 419, P 〈 0.01 ）. Conclusion The expression of PIBF requires allogeneic stimulation. The immunomodulatory effect of dydrogesterone is stronger than that of progesterone during implantation stage.