间日疟原虫抗原体外诱导间日疟患者PBMC凋亡的研究

Apoptosis of PBMCs induced in vitro by Plasmodium vivax antigen in patients with vivax malaria

目的分析间日疟原虫抗原(PvAg)诱导宿主外周血单个核细胞(PBMC)的凋亡水平方法采用流式细胞术结合应用膜联蛋白-V(Annexin-V),测定7例间日疟现症感染者PBMC经PvAg刺激后CD4+T细胞、CD8+T细胞及CD19+B细胞的凋亡率,并与空白对照组比较。结果经PvAg刺激后的间日疟现症感染者CD19+B细胞及CD4+T细胞凋亡率分别为7.26%和48.08%,与空白对照凋亡率4.08%和22.80%比较差异无统计学意义(P>0.05);CD8+T细胞凋亡率为55.16%,与对照凋亡率22.86%比较差异有统计学意义(P<0.05)。结论 PvAg可诱导间日疟现症感染者CD8+T细胞凋亡,从而可能参与介导间日疟原虫发生免疫逃逸。

Objective To investigate the level of PBMC apoptosis induced by Plasmodium vivax crude antigen（PvAg）during acute P.vivax infection.Methods PBMCs from 7 patients infected with P.vivax were incubated with PvAg.Next,the percentage of apoptotic CD4＋T cells,CD8＋T cells,and CD19＋ B cells was determined using flow cytometry（FCM） with annexin V and corresponding CD3 McAb.Results The percentage of apoptotic CD3＋CD8＋ T cells in PvAg-stimulated cells（55.16%） was significantly higher than that with spontaneous apoptosis observed in cultured cells without any stimulus（22.86%,P0.05）,but there were no significant differences in the percentages of apoptotic CD19＋ B cells and CD4＋T cells between PvAg-stimulated cells（7.26% and 48.08%,respectively） and spontaneously apoptotic cells（4.08% and 22.80%,respectively,P0.05）.Conclusion P.vivax induced the apoptosis of CD8＋ T cells,which may be partly responsible for the parasite＇s evasion of the immune response.