同一膀胱癌的三个亚细胞株和BIU-87膀胱癌细胞株的化疗药物敏感性研究

Chemosensitivity testing of three novel bladder transitional cell carcinoma lines derived from an untreated patient and BIU-87 cell line

用未经化疗的同一膀胱癌手术标本建立的三株膀胱癌细胞株 (BL Z2 11、BL S2 11、BL X2 11)和人膀胱移行细胞癌细胞株 BIU- 87进行了氟尿啶啶 (5- Fu)、顺氯氨铂 (CDDP)、和长春新碱 (VCR)的敏感性试验。结果表明 :四株膀胱癌细胞株对这三种化疗药物的敏感性存在差异。当药物浓度分别为其临床用量的计算值时 ,BL Z2 11细胞株对 5- Fu高度敏感 ,与其它三株细胞的敏感性存在显著差异(P <0 .0 5) ;四株细胞对 CDDP均敏感 ;对 VCR四株细胞均耐药。提示 :对化疗敏感性的差异不仅存在于不同的肿瘤病人 ,而且也存在同一肿瘤标本的不同细胞群体中。因此 ,化疗前进行药敏试验 ,实行用药个体化对提高疗效有重要作用。

The cytotoxicity of the cisplatin,vincristine and 5 fluorouracil on the three sub clonal human bladder transitional cell carcinoma cell lines (BLZ211,BLS211 and BLX211) derived from an untreated patient and BIU 87 bladder cancer cell line was evaluated using the MTT assay.4 lines showed different sensitivities to the 3 agents:when the concentrations of the drugs were equal to the concentration calculated in vivo.BLZ211 cells were more sensitive than the other lines to 5 Fu and the differences were statistically significant (P<0.05),all 4 cell lines were sensitive to CDDP and were not sensitive to VCE.Our results suggest that difference of chemosensitivity not only exists in bladder cancer patients but also occurs in the cell subpopulations from the same patient.The drug sensitivity testing of the clinical samples may have an important effect in chemotherapy.