COMT基因Met多态与子宫内膜异位症的关系

Study on the correlation between gene polymorphism of COMT and endometriosis

目的探讨COMT基因Met多态与子宫内膜异位症的关系。方法采用蛋白酶K消化-饱和酚氯仿法提取外周血白细胞基因组DNA,聚合酶链式反应-限制性片段长度多态性(PCR-RFLP)方法分别对120例子宫内膜异位症患者(内异症组,含59例腺肌病患者)和65例非内异症及腺肌病女性(对照组)进行COMT基因分型。结果 (1)内异症组和对照组G/G、G/A和A/A基因型频率分别为17.5%、43.3%和39.2%,9.2%、50.8%和40.0%,差异无统计学意义;内异症组和对照组A等位基因频率分别为27.1%和29.2%,两组比较差异无统计学意义;(2)G/A、A/A基因型个体发生子宫内膜异位症的相对危险度分别为G/G型个体的0.516倍(0.185-1.441,P>0.05)和0.450倍(0.165-1.232,P>0.05),差异无统计学意义。结论 COMT基因Met多态与子宫内膜异位症无相关性。

Objective To explore the correlation between gene polymorphism of Catechol-O-Methyltransferase（COMT） and endometriosis（EMs）.Methods Polymerase chain reaction and restriction fragment length polymorphism（PCR-RFLP） was used to analyze the polymorphism of COMT gene in 120 patients with endometriosis（59 with adenomyosis） and 65 healthy controls.Results There was no significant difference of the frequence of A allele and the distribution of genotype between EMs group and control group.The relative risk suffered from endometriosis of G/A and A/A genotype was 0.516（0.185-1.441） and 0.450（0.165-1.232） times of the G/G genotype respectively.Conclusion There was no correlation between the Met gene polymorphism of COMT and endometriosis.