黄皮素内酯Ⅱ在黄皮植物体的分布及对稗草生化代谢的影响

The distribution of 2′,3′-epoxyanisolactone in different organ of Clausena lansium(Lour.) Skeels and biochemical and metabolic effect on Echinochloa crusgalli L.

[目的]测定黄皮素内酯Ⅱ(2′,3′-epoxyanisolactone)在黄皮[Clausena lansium(Lour.)Skeels]植株的花、枝叶和果核中的含量,明确其对稗草生化代谢的影响。[方法]采用液相色谱法进行含量的测定,小杯法培养供试稗草。[结果]黄皮素内酯Ⅱ在花粗提物中的含量较少,仅为0.43%,在枝叶和果核中的含量分别为3.33%和3.21%。黄皮素内酯Ⅱ处理稗草植株,处理后稗草根中蛋白含量和氨基酸含量与未经处理存在明显的差异,在50μg/mL的浓度下,可以显著地降低稗草植株内的蛋白含量和增加植株内的氨基酸含量。当处理浓度为50、25、12.5、6.25、3.125μg/mL时,样品中的含氮量分别为1.402 8、1.317 2、1.250 2、1.128 7、0.903 2mg/g,比对照0.832 1mg/g均有所增加。黄皮素内酯Ⅱ处理后,稗草的蛋白含量与对照相比有所下降,处理浓度为50、25、12.5、6.25、3.125μg/mL时,蛋白含量比对照分别降低75.91%、66.43%、62.43%、58.57%和58.03%。[结论]黄皮素内酯Ⅱ对稗草代谢有一定影响。

[Objective]The content of 2′,3′-epoxyanisolactone in three different organ of Clausena dunniana were tested,the biochemical and metabolic effect on echinochloa crusgalli L.were investigated.[Method]The content of 2′,3′-epoxyanisolactone were analyzed by HPLC analyzing.bioassay of E.crusgalli was tested by small glass method.[Result]The result indicated that there are 0.43% in flower,3.21% in seeds and 3.33% in the leaf and bark,respectively.The result of assay on metabolism to E.crusgalli indicated that 2′,3′-epoxyanisolactone could cause the weakness of vigor and functions of root systems,and the decrease of total amount of protein of E.crusgalli,2′,3′-epoxyanisolactone could increase the quantity of amino acid,with the series of concentration of 2′,3′-epoxyanisolactone： 50 μg/mL,25 μg/mL,12.5 μg/mL,6.25 μg/mL and 3.125 μg/mL,the quantity of amino acid of controlled seeding were 1.402 8 mg/g,1.317 2 mg/g,1.250 2 mg/g,1.128 7 mg/g and 0.903 2 mg/g respectively,which was higher than the control at 0.832 1 mg/g.Protein quantity of controlled seeding of E.crusgalli decrease 75.91%,66.43%,62.43%,58.57% and 58.03% compared to the control respectively.[Conclusion]The result indicated that 2′,3′-epoxyanisolactone has influence on E.crusgalli.