儿宝颗粒对幼龄厌食大鼠模型CCK-8的调节作用

Erbao Granule: Adjusting CCK-8 in Juvenile Rats with Anorexia

目的 研究幼龄厌食大鼠中枢及外周八肽胆囊收缩素 (CCK - 8)的含量变化以及运脾方药对它们的调节作用。方法模拟小儿厌食症的主要病因制作幼龄厌食大鼠模型 ,用儿宝颗粒治疗 ,运用放免检测技术测定动物下丘脑及外周血中CCK -8的含量。结果 模型动物下丘脑和血浆CCK - 8浓度分别为 (12 2 .5 7± 31.79)pmoL/ g和 (5 0 6 .88± 113.32 ) pg/mL ,显著高于正常组 (89.15± 17.94) pmoL/g(P <0 .0 5 )和 (2 5 3.76± 6 5 .0 9) pg/mL(P <0 .0 5 ) ;摄食量明显下降 ,持续 3周以上 ;大小剂量治疗组摄食量均增加 ,下丘脑及血浆CCK - 8含量恢复正常。结论 幼龄厌食大鼠模型中枢和外周CCK - 8浓度增高 ,运脾方药对此有明显的抑制作用。

OBJECTIVE:To study the change of the contents of cholecystokini-octapeptide (CCK-8) in the central and peripheral areas in juvenile rats with anorexia and the effects of Erbao granule. METHOD:The juvenile rat model with anorexia was constructed by replicating the major causes of anorexia in children and was treated with Erbao granule. The contents of CCK-8 in the hypothalamus and plasma in rats were detected with RIA.RESULTS:The concentrations of CCK-8 in the hypothalamus and plasma of the model group were (122.57±31.79) pmoL/g and (506.88±113.32) pmoL/g, higher than the normal group (89.15±17.94)pmoL/g ( P <0.05) and (253.76±65.09) pmoL/g ( P <0.05). Their food intake was reduced for more than 3 weeks. In the treatment groups with either high or low dosage, the food intake was increased and the contents of CCK-8 resumed its initial reading. CONCLUSION:The concentrations of CCK-8 in the hypothalamus and plasma of the model rats can be increased and the therapy can significantly inhibit the increase.