一氧化氮与前列腺增生膀胱颈梗阻关系的研究

NITRIC OXIDE IN THE PROSTATIC TISSUES AND IT'S CORRELATION WITH THE DEVELOPMENT OF BLADDER OUTLET OBSTRUCTION SECONDARY TO BENIGN PROSTATIC HYPERPLASIA

目的：探讨一氧化氮（ＮＯ）与前列腺增生（ＢＰＨ）膀胱颈梗阻（ＢＰＯ）发病的关系。方法：应用双波长分光光度法，组织化学法检测２０例存在ＢＰＯ及１０例正常前列腺组织中的ＮＯ合酶（ＮＯＳ）活性及ＮＯＳ神经。结果：ＢＰＨ组织中的ＮＯＳ活性，ＮＯＳ神经的长度密度均较正常前列腺组织明显降低；ＮＯＳ活性，ＮＯＳ神经的Ｌｖ与ＢＰＨ患者的症状评分及反映ＢＰＯ客观指标的尿动力学参数相关。

Objective:To evaluate the relationship between the nitric oxide to the development of bladder outlet obstruction (BPO) secondary to benign prostatic hyperplasia (BPH).Methods:NO synthase (NOS) catalytic assay,NADPH diaphorase histochemistry were performed to investigate the NOS activity,NOS\|containing nerves of the 20 cases of BPH and 10 cases of normal prostatic tissues.Results:Both the level of the NOS activity and the length density (Lv) of NOS-containing nerve in the BPH tissues are markedly decreased compared with those in the normal prostatic tissues (P< 0.01 ).A statiscally significant correlation was observed between the level of NOS activity,the Lv of NOS-containing nerve and the international prostatic symtom score,maximum flow rate,maximum urethral pressure and maximum urethral closure pressure.No correlation was observed between the level of NOS activity,the Lv of NOS-containing nerve and the volume of prostatc.Conclusion:Our study showed that the development of BPH and its secondary BPO was related to the change of NOS activity and NOS-containing nerves in the prostatic tissues,which indieated that the reducing NO in the prostatic tissues might be one of the causes of BPO secondary to BPH.