摘要
借助正交设计法对白灵菇原生质体制备与再生进行研究.结果表明,原生质体最佳制备体系是:液体静置培养7d的菌丝体,在20g/L的溶壁酶作用下,以0.6mol/L蔗糖为渗透压稳定剂,28℃酶解2h,最高制备率为1.34×107个/mL;其最佳再生体系是:以液体静置培养7d的菌丝体,0.6mol/L MgSO4.7H2O为渗透压稳定剂,在15g/L溶壁酶与10g/L蜗牛酶作用下,34℃酶解3h,最高再生率为3.7%.
The conditions of preparing and regenerating of Pleurtus nebrodensis Lnzenga protoplasts were studied through orthogonal design experiments. The results showed that the optimal preparing conditions of Pleurtus nebrodensis Lnzenga protoplast were 7 - day-old mycelium cultured in static liquid, using 20 g/L Lywallzyme, applying 0.6 mol/L sucrose for osmotic pressure stabilizer, digesting at 28 ℃ for 2 hours. And protoplast achieved 1.34× 10^7/ml. Protoplasts produced in the conditions of 7 -day-old mycelium cultured in static liquid, applying 0.6 mol/L MgSO4 ·7H2O for osmotic pressure stabilizer, using 15 g/L Lywallzyme and 10 g/L Snailase, digesting at 34 ℃ for 3 hours were optimal to regeneration. And the regeneration rate was 3.7℃.
出处
《天津师范大学学报(自然科学版)》
CAS
2012年第1期88-92,共5页
Journal of Tianjin Normal University:Natural Science Edition
关键词
白灵菇
正交设计
原生质体
制备
再生
Pleurtus nebrodensis Lnzenga
orthogonal design
protoplast
preparation
regeneration