大黄素对狼疮性肾炎成纤维细胞生物学行为的影响

Effect of Emodin on Fibroblasts in Lupus Nephritis

目的：观察中药大黄素（ｅｍｏｄｉｎ）对狼疮性肾炎（ＬＮ）肾间质成纤维细胞生物学行为的影响。方法：将ＬＮ患者肾活检组织体外培养分离出成纤维细胞，分别用３Ｈ－ＴｄＲ掺入法和流式细胞术检测不同浓度的大黄素对人肾成纤维细胞增殖、凋亡及ｃ－ｍｙｃ蛋白水平表达情况。结果：大黄素能明显抑制人肾成纤维细胞的分裂增殖，并通过促使ｃ－ｍｙｃ蛋白高水平表达诱导细胞发生凋亡。结论：大黄素有抑制成纤维细胞增殖和促其凋亡的作用，从而达到减轻ＬＮ肾间质纤维化病变。

To observe the effects of emodin on human fibroblasts in culture of kidney in patients with lupus nephritis (LN). Methods: Fibroblasts were isolated from culture of kidney of LN patients, and the effect of emodin on 3H-TdR incorporated rate of fibroblasts was observed. The apoptosis and c-myc gene expression were detected in the same time by flow cytometry. Results: Emodin could inhibit the proliferation of fibroblasts, and promote the programmed cell death through upregulate c-myc gene expression in human renal fibroblasts. Conclusion: Emodin can inhibit proliferation and promote apoptosis of fibroblasts, which may be important in ameliorate interstitial fibrosis, thus improving prognosis of LN.