摘要
目的:观察中药大黄素(emodin)对狼疮性肾炎(LN)肾间质成纤维细胞生物学行为的影响。方法:将LN患者肾活检组织体外培养分离出成纤维细胞,分别用3H-TdR掺入法和流式细胞术检测不同浓度的大黄素对人肾成纤维细胞增殖、凋亡及c-myc蛋白水平表达情况。结果:大黄素能明显抑制人肾成纤维细胞的分裂增殖,并通过促使c-myc蛋白高水平表达诱导细胞发生凋亡。结论:大黄素有抑制成纤维细胞增殖和促其凋亡的作用,从而达到减轻LN肾间质纤维化病变。
To observe the effects of emodin on human fibroblasts in culture of kidney in patients with lupus nephritis (LN). Methods: Fibroblasts were isolated from culture of kidney of LN patients, and the effect of emodin on 3H-TdR incorporated rate of fibroblasts was observed. The apoptosis and c-myc gene expression were detected in the same time by flow cytometry. Results: Emodin could inhibit the proliferation of fibroblasts, and promote the programmed cell death through upregulate c-myc gene expression in human renal fibroblasts. Conclusion: Emodin can inhibit proliferation and promote apoptosis of fibroblasts, which may be important in ameliorate interstitial fibrosis, thus improving prognosis of LN.
出处
《中国中西医结合杂志》
CAS
CSCD
北大核心
2000年第3期196-198,共3页
Chinese Journal of Integrated Traditional and Western Medicine
基金
广东省医学科研基金