芪麝颈康方对兔退变颈椎骨赘形成部位碱性磷酸酶活性的影响

Experimental Study on Effect of Qishejingkang Recipe on Activity of Alkaline Phosphatases in Osteophyte Formation of Degenerated Cervical Vertebrae of Rabbits

目的：观察芪麝颈康方对退变颈椎骨赘形成部位碱性磷酸酶活性的影响。方法：选用１０月龄新西兰兔，通过切除动物Ｃ２～Ｃ７棘上韧带、棘间韧带和分离椎旁两侧肌肉，建立颈椎退行性改变的动物模型。组织学观察颈椎椎体骨赘形成的部位，采用生物化学法分别检测颈椎间盘中软骨终板、髓核和纤维环中碱性磷酸酶的活性。结果：证实骨赘来源于软骨终板，中药组退变椎间盘软骨终板中碱性磷酸酶的活性明显低于模型组（Ｐ＜０．０１）。结论：芪麝颈康方可降低骨赘生长部位的碱性磷酸酶活性，推测芪麝颈康方有抑制骨赘形成的作用。

To study the effect of Qishejingkang recipe (QSJKN) on activity of alkaline Phosphates (ALP) in osteophyte formation of degenerated cervical vertebrae. Methods: A model of degenerated cervical vertebrae in the rabbit was established through resection of the cervical supraspinous and interspinous ligaments from the cervical spine elicited by surgical intervention. The sites of osteophyte was observed with a light microscope. The activity of ALP of the annuls fibrosis and nucleus pulpous, end-plate in each cervical spine was assayed with biochemical method respectively. Results: Osteophyte come from end-plate, the activity of ALP in the degenerated end-Plate in QSJKR group declined significantly Compared with mode group. Conclusion: QSJKR could decrease the activity of ALP in end-plate of degenerated cervical vertebrae and it might inhibit the formation of osteophyte.