慢性牙周炎患者血浆β-防御素-2浓度的变化

The Change of Plasma Beta-defensin-2 Concentration in Patients with Chronic Periodontitis

目的:观察慢性牙周炎患者血浆β-防御素-2浓度的变化,揭示其与慢性牙周炎发病的相关性。方法:收集慢性牙周炎患者和同期牙周健康者各80例。ELISA检测血浆β-防御素-2浓度。统计分析其与慢性牙周炎发病的相关性。结果:经t检验,慢性牙周炎患者血浆β-防御素-2浓度(405.2±133.9)pg/ml显著低于牙周健康者(641.6±128.3)pg/ml(t=6.492,P<0.01)。经Spearman相关分析,慢性牙周炎患者血浆β-防御素-2浓度与牙周探诊深度(r=-0.482,P<0.01)、附着丧失(r=-0.498,P<0.01)和出血指数(r=-0.501,P<0.01)均呈显著负相关。Logistic回归分析显示,血浆β-防御素-2浓度(OR=0.415,95%CI=0.297～0.862,P<0.01)是罹患慢性牙周炎的独立危险因素。ROC曲线分析显示,血浆β-防御素-2浓度预测罹患慢性牙周炎有显著预测价值(曲线下面积=0.843,95%CI=0.774～0.894,P<0.01),且判定血浆β-防御素-2浓度<621.5 pg/ml,对预测罹患慢性牙周炎有81.3%的灵敏度和75.0%的特异度。结论:慢性牙周炎患者血浆β-防御素-2水平降低,血浆β-防御素-2水平降低可能是慢性牙周炎的危险因素。

Objective To observe the change of plasma beta-defensin-2 level was determined in patients with chronic periodonti- tis and its correlation with disease was evaluated. Methods 80 consecutive patients with chronic periodontitis and 80 healthy controls were enrolled into this study. Plasma beta-defensin-2 concentration was measured by ELISA. Its Correlation with disease was analyzed statistically. Results The plasma beta-defensin-2 level （405.2 ±133.9 pg/rnl） in patients was statistically significantly lower than that （641.6 ± 128.3 pg/ml） in healthy controls using t test （t =6. 492, P 〈0.01 ）. Spearman correlation coefficient analysis showed that probing depth （ r = - 0.482,P 〈 0.01 ）, attachment loss （ r = = 0.498,P 〈 0. 01 ） and bleeding index （ r = - 0.501, P 〈 0. 01 ） were negatively related to plasma beta-defensin-2 level in the patients with chronic periodontitis. On a multivariate logistic regression, plasma beta-defensin-2 level （ OR = 0.415, 95% CI = 0.297±0.862, P 〈 0.01 ） was an independent variable predicting chronic periodontitis. A receiver operating characteristic curve identified that a plasma beta-defensin-2 level 〈 62l. 5 pg,/ml predicted chronic peri- odontitis with 81.3 % sensitivity and 75.0% specificity （ area under curve = 0. 843, 95% CI = 0.774 - 0. 894, P 〈 0.01 ） respectively. Conclusion Deereased beta-defensin-2 was found after chronic periodontitis and low beta-defensin-2 may be independently associated with chronic periodontitis as well as a risk factor.