摘要
目的观察针刺预处理及不同配穴对肝郁脾虚证大鼠模型的防治效应,探讨其可能的作用机理。方法将50只雌雄各半wistar大鼠随机分为5组,每组雌雄各半,分开喂养。分组:①正常对照组10只;②模型对照组10只;③"太冲+三阴交"组10只;④"太冲+三阴交+天枢"组10只;⑤"太冲+三阴交+上巨虚"组10只。对针刺组进行7d的电针预处理后,对除正常对照组以外的大鼠使用慢性应激方法(不可预知)造模15d。采用ELISA方法,观察各组大鼠血清中VIP、GAS含量变化。结果与空白对照组相比,模型对照组血清血管活性肽(VIP)、胃泌素(GAS)含量下降,变化具有统计学意义(P<0.05);与各针刺预处理不同配穴组比较,模型对照组血清VIP、GAS含量下降,变化具有统计学意义(P<0.05);与空白对照组相比,各针刺预处理不同配穴组血清VIP、GAS含量波动未见明显统计学意义。结论电针预处理肝郁脾虚证大鼠可以调节大鼠血清中VIP、GAS含量,这可能是针刺预处理方法防治肝郁脾虚证的机制之一。
Objective To observe acupuncture point pretreatment and different combination of points on VIP and GAS of liver depression and spleen deficiency rats, and discuss the possible mechanism. Methods:60 wistar rats were divided into 5 groups, which were normal controls, Model control group,taichong + sanyinjiao group, taichong + sanyinjiao + tianshu group, taichong + sanyinjiao + shangjuxu group. After 7 days' acupuncture pretreatment on acupuncture groups, we use chronic stress method (umpredietable) to made the model for 15 days. Results:Compared with the control group, Model control group's serum VIP, GAS down the content, the change had a statistical significance( P 〈 O. 05 ) ;Compared with the acupuncture pretreatment groups, Model control group' s serum VIP, GAS down the content, the change had a statistical significance(P 〈 0.05) ;Compared with the control group, acupuncture pretreatment groups' serum VIP, GAS did not have a statistical significance. Conclusion: Electro- acupuncture pretreatment can adjust VIP, GAS content in serum. This may be a mechanism that acupuncture pretreatment method can prevention and cure liver depression and spleen deficiency.
出处
《光明中医》
2012年第2期309-311,共3页
GUANGMING JOURNAL OF CHINESE MEDICINE
基金
辽宁省科技厅自然基金资助(No.L2010367)