摘要
目的研究肺癌中增殖细胞核抗原表达的意义。方法应用抗增殖细胞核抗原（抗ＰＣＮＡ）单克隆抗体，采用链菌素亲生物素蛋白－过氧化酶连接（Ｓ－Ｐ）免疫组化方法对３２例患者肺癌细胞中ＰＣＮＡ表达进行检测。结果ＰＣＮＡ在肺癌组织中的表达与肺癌的分级、分期里正相关（Ｐ＜０．０５），有淋巴结转移组ＰＣＮＡ的表达率高于没有淋巴结转移组（Ｐ＜０．０５）。获随访的１６例结果显示ＰＣＮＡ高表达与死亡率呈显著负相关。结论检测肺癌组织中ＰＣＮＡ的表达对肺癌的分级、分期、预后以及生物学行为有重要意义。
Objective To study the expression and its significance of proliferation cell nuclear antigen (PCNA) in patients with lung cancer. Methods The expressions of PCNA in 32 cases of lung cancer were measrued by McAb of anti-PCNA and streptostacin-peroxidase (S-P ) immunohisto chemical method. Results The exprssion of PCNA was postively related to the grade and phase of lung cancer (P<0. 05), and, in lymphaden metastasis group higher than that in non-lymphaden metastasis group (P<0. 05). The high expression of PCNA is negtatively related to mortality. Conclusion The expression of PCNA is significant for the grade, phase, prognosis and biological behavior of lung cancer.
出处
《临床内科杂志》
CAS
2000年第2期107-108,共2页
Journal of Clinical Internal Medicine
关键词
肺肿瘤
增殖细胞核抗原
单克隆抗体
免疫组化
Lung neoplasma
Proliferation cell nuclear antigen
Monoclonal antibody
Immunohistochemistry method
