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低体系扩增技术在法医学中的应用综述 被引量：1

Application of low volume PCR in the forensic science
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摘要 降低扩增体系是提高微量DNA扩增灵敏度的手段之一。本文主要介绍了低体系扩增技术及其在国内外法医学领域应用的研究进展,并对低体系扩增技术解决法医学难题的应用前景进行初步探讨。 In this article, the low volume PCR （LV-PCR） technology and its applications in forensic science were introduced, and the prospect of LV-PCR for solving some difficult problem in forensic science was also discussed.
作者 亓冰 张平 张家栋 钱静 俞卫东 胡兰
机构地区 江苏省南京市公安局浦口分局 南京医科大学法医系 江苏省南京市公安局刑事科学技术研究所 公安部物证鉴定中心
出处 《刑事技术》 2012年第1期46-48,共3页 Forensic Science and Technology
关键词 低体系扩增技术 法医学 应用 综述 LV-PCR forensic medicine application prospect
分类号 D919.4 [医药卫生—法医学]
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  • 1顾丽华,张晨,陈连康,郑会芬,程莉,周怀谷.激光显微捕获口腔上皮细胞的DNA分型[J].法医学杂志,2006,22(3):196-197. 被引量：11
  • 2余茂効 译.分子克隆操作指南[M].北京:科学技术出版社,1986.279-282.
  • 3王晓兰.有机化学[M].北京:人民教育出版社,1979.57-63.
  • 4PE 公司. AmpFlSTR Profiler plusTM PCR Amplification kit User's Manual[ S]. PE Corporration 1998.
  • 5Hanson EK, Ballantyne J. Whole genome amplification strategy for forensic genetic analysis using single or few cell equivalents of genomic DNA. Anal Biochem , 2005 , 346: 246-257.
  • 6Gill P,Whitaker J,Flaxman C, et al. An investigation of the rigor of interpretation rules for STRs derived from less than 100 pg of DNA.Forensic Sci Int, 2001, 112: 17-40.
  • 7Sun G, Kaushal R, Pal P, et al. Whole-genome amplification:relative efficiencies of the current methods. Leg Med. 2005, 7 : 279-286.
  • 8Nelson DL, Ledbetter SA, Corbo L, et al. Mu polymerase chain reaction: a method for rapid isolation of human-specific sequences from complex DNA sources. Proc Natl Acad Sci U S A, 1989, 86:6686-6690.
  • 9Telenius H, Carter NP, Bebb CE, et al. Degenerate oligonucleotide-primed PCR: general amplification of target DNA by a single degenerate primer. Genomics, 1992, 13 : 718-725.
  • 10Kittler R, Stoneking M, Kayser M. A whole genome amplification method to generate long fragments from low quantities of genomic DNA. Anal Biochem, 2002, 300: 237-244.

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  • 1冯雪飞,胡兰,张惠芹,李鑫,刘晓.显微操作方法捕获精子细胞及对其DNA分型结果研究[J].中国人民公安大学学报（自然科学版）,2006,12(2):34-38. 被引量：7
  • 2Bianconi E, Piovesan A, Facchin F, et al. An estimation of the num- ber of cells in the human body :J]. Ann Hum Biol, 2013,40(6):463- 471.
  • 3Budowle B, Onorato A J, Callaghan TF, et al. Mixture inter- pretation: defining the relevant features for guidelines for the assessment of mixed DNA profiles in forensic casework Eli . J Forensic Sci, 2009,54(4):810-821.
  • 4Gill P, Brown RM, Fairley M, et al. National recommendations of the technical UK DNA working group on mixture interpreta- tion for the NDNAD and for court going purposes [J]. Forensic Sci Int Genet, 2008,2(1):76-82.
  • 5Li CX, Qi B, Ji AQ, et al. The combination of single cell mi- cromanipulation with LV-PCR system and its application in forensic science Eli. Forensic Sci Int Genet Supplement Series, 2009,2(1):516-517.
  • 6lardis ER. A decadd s perspective on DNA sequencing technol- ogy Eli. Nature, 2011,470(7333): 198-203.
  • 7Navin N, Kendall J, Troge J, et al. Tumour evolution inferred by single-cell sequencing Eli. Nature, 2011,472(7341):90-94.
  • 8Di Martino D, Giuffre G, Staiti N, et al. Single sperm cell isola- tion by laser microdissection :J]. Forensic Sci Int, 2004, 2(146 Suppl):S151-153.
  • 9Sanders CT, Sanchez N, Ballantyne J, et al. Laser microdissection separation of pure spermatozoa from epithelial cells for short tandem repeat analysis [J]. J Forensic Sci, 2006,51(4):748-757.
  • 10Li CX, Wang GQ, Li WS, et al. New cell separation technique for the isolation and analysis of cells from biological mixtures in forensic caseworks [J:. Croat Med J 2011,52(3):293-298..

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2012年 第1期

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