摘要
目的考察用合煎、分煎方法制备的半夏厚朴汤汤剂中和厚朴酚含量的变化。方法建立高效液相色谱方法,采用Diamonisl C18柱(200 mm×4.6 mm,5μm),流动相为甲醇-水(78∶22),流速为1 mL/min,检测波长为294 nm。分析半夏厚朴汤合煎液和分煎液样品中和厚朴酚的含量变化。结果半夏厚朴汤分煎液和合煎液中和厚朴酚含量无差异性。结论合煎液和分煎液中和厚朴酚的含量在煎煮过程中没有因素的影响。
Objective To investigate the honokiol contents in mixed decocting and separated decoction of Banxia Houpu Decoction. Methods The HPLC method was established with the Diamonisl C18 column (200 mm ×4. 6 mm,5μm),the mobile phase of methanol-water (78 : 22),the flow rate of 1 mL/min and the detection wavelength of 294 nm in order to determine the contents of honokiol. Results The average honokiol content of separated decoction had no difference with that of mixed decoction. Conclusion Comparing two kinds of decoction,the honokiol content of mixed decoction and separated decoction is not affected by the boiling process factors.
出处
《中国药业》
CAS
2012年第4期21-22,共2页
China Pharmaceuticals
基金
遵义市中药现代化科技产业研究开发专项项目课题
项目编号:市科合中药专字〔2009〕4号
关键词
半夏厚朴汤
和厚朴酚
合煎
分煎
高效液相色谱法
Banxiahoupu Decoction
honokiol
mixed decoction
separated decoction
HPLC