毛细管气相色谱法测定克痢痧胶囊中丁香酚含量

Determination of Eugenol in Kelisha Capsules by GC

目的建立用于测定克痢痧胶囊中丁香酚含量的气相色谱法,以提高原质量标准。方法选用HP innowax弹性石英毛细管柱(30 mm×0.32 mm,0.25μm);检测器为FID检测器;柱温为程序升温,初始温度为100℃,以10℃/min速率升至200℃,保持5 min;气体流量为1.2 mL/min(载气为氮气);分流进样,分流比为5∶1,进样量为1μL。结果丁香酚的进样量线性范围为0.072 6～1.21μg(r=0.999 9),平均加样回收率为97.62%,RSD=0.56%(n=6)。结论该测定方法简便、快速,准确性和重复性好,可用于克痢痧胶囊的质量控制。

Objective To develop a GC method for determining the content of eugenol in Kelisha Capsules to control the quality better. Methods The sample was analyzed by HP Innowax column（30 mm × 0.32 mm,0.25 μm）with FID detector. The initial temperature was set at 100 ℃ ,then risen to 200 ℃ with the rate of 10 ℃/min by temperature programming and kept for 5 rain. The flow rate was at 1.2 mL/min. The split ratio was 5 ： 1,the injection volume was 1 μL. Results The linear range of eugenol was 0. 072 6 - 1.21 μg （r=0.999 9）.The average recovery rate was 97.62% and RSD was 0.56% （n=6）.Conclusion The method is simple,rapid and accurate with good reproducibility, which can be used for quality control of this preparation.