摘要
[目的]探讨生长激素释放肽（Ghrelin）对高脂环境下大鼠成肌细胞糖代谢的影响,为深入研究2型糖尿病的发病机制和防治提供理论依据.[方法]在给予原代培养大鼠成肌细胞0.3 mM棕榈酸（PA组）处理12 h的同时用加入10-11 M、10-9 M、10-7 M Ghrelin（干预组）,用同位素示踪法检测葡萄糖摄取,分别用RT-PCR和Western blotting法检测GLUT4 mRNA表达和蛋白水平.[结果]与对照组相比,在0.3 mM棕榈酸的PA组中,葡萄糖摄取明显下降,但是GLUT4 mRNA表达和蛋白水平未见明显改变.与0.3 mM的PA组对比,随着Ghrelin干预浓度的逐渐增加,葡萄糖摄取也逐渐增加,并呈剂量反应关系,其中10-7M Ghrelin组葡萄糖摄取增加最为明显,然而GLUT4 mRNA表达和蛋白水平在各Ghrelin剂量组中未见明显改变.[结论]Ghrelin可增加高脂环境下大鼠成肌细胞的糖摄取,并且存在剂量依赖关系,而未见对GLUT4产生影响.
[Objective] To explore the effect of ghrelin on glucose metabolism in rat myoblast in high fat environment in order .to provide theoretical basis for further studying the prevention and pathogenesis of type 2 diabetes mellitus. [Methods]The rat myoblast in primary culture was treated with 0.3mM palmitic acid for 12h, meanwhile added with 10-11 M, 10-9 M and 10-7 M Ghrelin. Isotope tracer method was used to detect glucose uptake. RT-PCR and Western blotting were used to detect the expression of GLUT4 mRNA and pro- tein level. [Results] Compared with the control group, glucose uptake in 0. 3mM PA group obviously de- creased. However, there was no obvious change of GLUT4 mRNA expression or protein level. Compared with 0.3mM PA group, glucose uptake gradually increased with the increasing of ghrelin in a dose-response man- ner. The increasing of glucose uptake in 10^-7 ghrelin group was the most obvious. The expression of GLUT4 mRNA and protein level had no obvious change in different doses of ghrelin groups. [Conclusion] Gh- relin can increase glucose uptake in rat myoblast in high fat environment in a dose-response manner, and has no influence on GLUT4.
出处
《医学临床研究》
CAS
2012年第1期8-10,14,共4页
Journal of Clinical Research
基金
[基金项目]辽宁省教育厅科研项目（L2010598）
