海洋来源真菌Hypocrea virens菌丝体的化学成分研究

Study on the Chemical Constituents from the Mycelium of Marine-Derived Fungus Hypocrea Virens

采用硅胶柱色谱、Sephadex LH-20凝胶柱色谱、开放ODS柱色谱以及HPLC等对海洋来源真菌Hypocreavirens的菌丝体进行化学成分分离,通过理化性质和波谱数据鉴定化合物的结构。从菌丝体的丙酮提取物中分离得到8个化合物,分别鉴定为1(10→6)Abeo-(22E,24R)-ergosta-5,7,9,22-tetraene-3α,11α-diol(Ⅰ)、24-甲烯基羊毛脂烷-8-烯-3β-醇(Ⅱ)、(22E,24R)-麦角甾-5,7,22-三烯-3β-醇(Ⅲ)、(22E,24R)-麦角甾-8,22-二烯-3β,5α,6β,7α-四醇(Ⅳ)、5α,9α-过氧-(22E,24R)-麦角甾-7,22-二烯-3β,6β-二醇(Ⅴ)、5α,8α-过氧-(22E,24R)-麦角甾-6,22-二烯-3β-醇(Ⅵ)、(22E,24R)-麦角甾-7,22-二烯-3β,5α,6β-三醇(Ⅶ)、3β,5α,9α-三羟基-(22E,24R)-麦角甾-7,22-二烯-6-酮(Ⅷ),其中化合物Ⅰ、Ⅱ、Ⅳ、Ⅴ、Ⅷ均为首次从该属真菌中分离得到,化合物Ⅲ、Ⅵ、Ⅶ为首次从该种真菌中分离得到。

Eight compounds were isolated from the mycelium of marine-derived fungus Hypocrea virens by using silica gel,Sephadex LH-20, open ODS column chromatography and HPLC. On the basis of spectral data and physicochemical properties, their structures were identified as 1 （10→6） abeo- （22E, 24R）-ergosta-5,7,9,22- tetraene-3a, 1 la-diol （ I ）, 24-methylenelanostane-8-ene-3 β-ol （ II ）, （ 22E, 24R）-ergosta-5,7,22-triene-3β-ol （ III ）, （22E, 24R）-ergosta-8,22-diene-3β, 5a, 6β, 7a-tetraol （ IV ）, 5a, 9a-epidioxy- （ 22E, 24R）-ergosta-7,22-diene-3β, 6β- diol（ V ）, 5a, 8a-epidioxy-（22E, 24R）-ergosta-6,22-diene-3β-ol （ VI ）, （22E, 24R）-ergosta-7,22-diene-3β, 5a, 6β-tri- ol（VII） ,3β,5a,9a-trihydroxy-（22E,24R）-ergosta-7,22-diene-6-one（VIII）. Compounds I , II , IV, V , VII were isolated from the Hypocrea genus of marine-derived fungus for the first time,and compounds III, VI, VII were isolated from the marine-derived fungus Hypocrea virens for the first time.