低营养产絮菌Rheinheimera aquimaris P6的分离筛选及其絮凝特性研究

Screening and Flocculation Performance of A Bioflocculant-Producing Strain Rheinheimera Aquimaris P6 Cultured in Low Nutrition Medium

采用低营养培养基从海洋底泥中分离筛选出一株高效产絮菌Rheinheimera aquimaris P6。该菌株在碳源为1.5 g.L-1葡萄糖、氮源为1.5 g.L-1尿素、发酵pH值为7、投加0.2 g.L-1 Fe2+的最佳培养条件下,所产絮凝剂的絮凝率达90.2%;金属离子Fe2+、Mg2+对其产絮能力有促进作用;通过紫外光谱、蛋白和糖显色反应、红外光谱等手段分析确定絮凝剂的主要成分为多糖和蛋白质(1∶1)。研究表明Rheinheimera aquimaris P6是一株产絮性能稳定的高效低营养产絮菌,为从源头降低絮凝剂生产成本、推动我国生物絮凝剂的工业化应用提供了重要的信息。

An high-efficiency bioflocculant-producing strain Rheinheirnera aquimaris P6 was screened from marine sediment using low nutrition medium. The flocculation rate reached 90.2% adding 0.2 g . L-1 Fe2＋ ,u- sing 1.5 g . L -1 glucose as carbon source,1.5 g . L-1 urea as nitrogen source at the pH value of 7. Besides,metal ions such as Fe2＋ , Mg2＋ could accelerate the bioflocculant-producing performance. UV Spectrum, chromogenic reaction for protein and sugar,IR spectrum analysis determined that the main components of the bioflocculant were polysaccharides and protein with a ratio of 1 ： 1. The experimental results indicated that Rheinheimera aquirnaris P6 was a stable and high-efficiency bioflocculant-producing strain. The results provided important in- formation for reducing flocculant production cost from source and promoting the industrial bioflocculant application.