Cyclin D1在乳腺浸润性小叶癌的表达及其意义

Expression of Cyclin D1 in Invasive Lobular Carcinoma of the Breast and Its Significance

目的探讨Cyclin D1在乳腺浸润性小叶癌中的表达及其意义。方法采用免疫组化染色方法对75例具有经典型乳腺浸润性小叶癌结构的标本进行Cyclin D1免疫组化检测。结果 Cyclin D1阳性表达率为93.33%(70/75),其中41例(54.67%)呈弥漫强阳性表达(3+,6～7分),21例(28%)呈中阳性表达(2+,4～5分),8例(10.67%)呈弱阳性表达(1+,2～3分);仅有5例(6.67%)呈阴性表达(0～1分)。Cyclin D1的阳性表达在患者年龄是否≥50岁、肿瘤最大径是否≥2cm、淋巴结是否有转移、是否累及乳头间质及其下大导管、雌孕激素受体有无及P53是否突变等因素的分组中无统计学差异(P>0.05);而CyclinD1阳性表达程度与淋巴结是否有转移具有一定的负相关性(P<0.05)。结论 Cyclin D1在乳腺浸润性小叶癌中呈过表达状态,该蛋白过表达提示其在基因水平上可能出现了某种程度的异常,这一假设尚需进一步行基因检测加以证实。

Objective To investigate the expression and its significance of Cyclin D1 in invasive lobular carcinoma （ILC） of the breast. Methods The specimens from 75 cases of ILC were detected using immuno- histochemieal method. Results Of these 75 cases, Cyclin D1 was positive on the nucleus in 70 cases （93.33%）, among which 41 （54.67%） showed strong diffuse staining （3 ~, 6-7 points）, and 21 （28%） were moderately positive （2 ＋, 4-5 points）, and 8 （ 10.67% ） were weak positive （ 1 ＋, 2-3 points）. Only 5 （6. 67% ） cases showed negative results （0-1 points）. The positive expression of Cyclin D1 showed no sig- nificant differences among patients in terms of age （ 〉 50 years） , maximum tumor diameter （ 〉 2cm） , lymph node metastasis, involvement of papillary interstitial tissue and the large duct beneath it, existence of estrogen and progesterone receptors, and the possible mutation of P53. However, an inverse correlation was found be- tween the degrees of the positive staining of Cyelin D1 and the lymph node metastasis （ P 〈 0. 05 ）. Conclusion Cyclin D1 is overexpressed in ILC, suggesting that there may be certain genetic abnormalities.