2010年临沂市部分健康人群手足口病隐性感染调查

Survey on Recessive Infection Status of Hand-foot-mouth Disease in Linyi City,2010

[目的]调查临沂市健康人群中肠道病毒的隐性感染情况,追踪手足口病流行的传播因素,为制定手足口病预防和控制策略提供科学的依据。[方法]采用多阶段整群抽样方法,在临沂市选择2009年手足口病发病率高发县区和低发县区的城镇和农村托幼机构各一处,于4、6、9月各采集儿童及其监护人大便标本,用荧光定量PCR进行肠道病毒核酸检测。[结果]合计检测1 499人,阳性159人,阳性检出率为10.61%。阳性检出率,4月为7.50%、6月为15.06%、9月为9.31%(P<0.05);农村村民为9.20%,托幼儿童为12.56%;兰山区为13.12%,平邑县为7.35%(P<0.05);男性为12.67%,女性为10.26%。[结论]在健康人群中存在肠道病毒隐性感染情况,隐性感染率的高低与我市手足口病的地区分布、季节性变化是一致的。

[Objective]To survey the latent infection rate of enteroviruses among healthy people in Linyi city, track the spread factors of HFMD （hand-foot-mouth disease）,so as to provide the scientific basis for implementing prevention and control strategy of HFMD. [Methods]Cross-seetional study was performed 3 times in April,June, September respectively with multi-stage cluster sampling method. One HFMD high-risk county and one low risk county of 2009 was chosen firstly, and then one urban kindergarten and one rural kindergarten was selected in each county. Fecal specimens were collected from children and their guardians. The fluorescent quantitative PCR was used for the detection of enterovii：us in fecal spec- imens. [Results]Totally 1 499 fecal specimens were collected in this study,positive for 159 people,the positive rate of in- fection for 10.61%, negative infection rate of 89.39%. In April 7.50%, In June 15.06%, In September 9.31% （P 0.05） ;Rural village 9.20% ,the kindergarten children of 12.56% ;Lanshan District 13.12% ,Pingyi county is 7.35% （ P 〈0.05） ;12.67% in boys, and 10.26 % in girls, [Conclusion]Recessive infection of enteroviruses exists in the healthy peo- ple＇ and the levels of infection rate are consistent with the distributions of HFMD by people, time and place （commonly known as three-dimension distribution）.