过氧化物酶体增殖物激活受体-γ在炎症性肠病中的表达及意义

Expression of peroxisome proliferator activated receptor gamma in intestinal tissue in patients with inflammatory bowel disease

目的观察过氧化物酶体增殖物激活受体-γ(PPAR-γ)在炎症性肠病(IBD)中的表达,探讨其可能的意义。方法采用免疫组织化学方法检测28例克罗恩病(CD)患者、38例溃疡性结肠炎(UC)患者及30例健康对照肠道组织中PPAR-γ蛋白的表达,并进一步分析PPAR-γ蛋白与IBD临床特征的关系。结果 PPAR-γ在UC患者中表达阳性率为34.21%(13/38),明显低于正常对照63.33%(19/30)及CD患者60.71%(17/28)(P<0.05);PPAR-γ蛋白表达与UC疾病活动性明显相关(P<0.05),与UC严重程度、病变部位及CD临床特征无明显相关(P>0.05)。结论 PPAR-γ蛋白在UC肠道组织中表达降低,且与疾病活动性明显相关。提示PPAR-γ可能在UC发生、发展中发挥重要作用。

Objective To investigate the expression and clinical significance of PPAR-γ in intestinal tissue in patients with IBD. Methods Twenty eight patients with Crohn＇s disease （CD）, thirty eight patients with ulcerative colitis （UC）and thirty normal controls were enrolled. Protein expression of PPAR-γ in IBD intestinal tissue and normal controls was determined hy immunohistochemical staining, and its relationship with clinical characteristics was analyzed. Results Compared with normal controls and CD patiants, protein expression of PPAR-γ in intestinal tissue in UC patients was significantly decreased （34.21 vs. 63.33 % and 60.71 % respectively, P〈0.05） ; The expression of PPAR-γ was significantly related with disease activity in UC patients（P〈0.05）, but not related with severity of disease, site of lesions and CD clinical characteristics. Conclusion Protein expression of PPAR-γ is low in UC patients, and was significant correlate with disease activity, which suggest that PPAR-γ may play an important role in the pathogenesis of UC.