三角褐指藻细胞破碎方法的研究

Research on the Methods of Phaeodactylum tricornutum Cells Fragmentation

三角褐指藻是一种含有较高多不饱和脂肪酸的海洋单胞藻。为了从细胞中提取不饱和脂肪酸,采用反复冻融法、超声波破碎法和匀浆法分别对三角褐指藻细胞进行破碎,并测定油脂提取率。结果表明,超声波破碎法、反复冻融法和匀浆法的最大细胞破碎率分别为91.5%,56.7%和81.2%;油脂提取率分别为18.1%,13%和13.4%。从操作的简捷性和经济效益来看,超声波破碎法优于反复冻融法和匀浆法。

The marine diatom Phaeodactylum tricornutum is rich in polyunsaturated fatty acids（PUFAs）.In order to extract PUFAs from cells,three methods of cells fragmentation,including ultrasonic method,freezing-thawing method and homogenate method are introduced.The result shows that cell disruption rates are 91.5%,56.7% and 81.2% respectively; extraction rates are 18.1%,13% and 13.4%.Considering the operating convenience and economic benefits,the ultrasonic method is superior to the methods of freezing-thawing and homogenate.