去铁酮对铝染毒大鼠脑组织中甘氨酸和γ-氨基丁酸及铝和必需元素的影响

Effect of Defriprone on Glycine and γ-amino-butyric-acid and Essential Elements in Brain of Rats Exposed to Aluminum

目的探讨去铁酮(defriprone,DFP)对铝染毒大鼠脑组织中甘氨酸(Gly)、γ-氨基丁酸(GABA)两种抑制性氨基酸类神经递质及铝和必需元素影响。方法将35只健康清洁级Wistar雄性大鼠按体重随机分为5组,分别为阴性对照组和铝单独染毒组以及铝+低、中、高剂量DFP组,每组7只,采用灌胃方式染毒,每天1次,每周连续染毒5 d,间隔2 d。前8周,阴性对照组给予生理盐水(1 ml/d),其他4组给予AlCl3溶液(354.7 mg/kg)。后2周,阴性对照组与铝染毒组给予生理盐水(1 ml/d);铝+DFP低、中、高剂量组分别给予不同浓度DFP溶液(13.72、27.44和54.88 mg/kg),每天1次。测定脑组织中Gly、GABA及铝、铜、镁、钙和铁的含量。结果与阴性对照组比较,铝单独染毒组和铝+各剂量DFP组大鼠脑组织中Gly和GABA的含量均较高,差异有统计学意义(P<0.01)。与铝单独染毒组比较,铝+各剂量DFP组大鼠脑组织中Gly和GABA的含量均较低,差异有统计学意义(P<0.01)。与阴性对照组比较,铝单独染毒组和铝+低、中剂量DFP组大鼠脑组织中铝含量均较高,差异有统计学意义(P<0.01)。与铝单独染毒组比较,铝+各剂量DFP组大鼠脑组织中铝含量均较低,差异有统计学意义(P<0.01);且随着DFP剂量的升高,大鼠脑组织中的铝含量呈下降趋势。各组大鼠脑组织中铜、镁、钙和铁含量间比较,差异均无统计学意义。结论 DFP可通过促进铝排出,恢复铝染毒大鼠脑组织中Gly和GABA的正常代谢,且对铜、镁、钙、铁等必需元素在脑中平衡不产生影响,对中枢神经系统具有明显保护作用。

Objective To investigate the effects of defriprone （DFP） on Gly and GABA and contents of aluminum and essential elements in brains of rats exposed to aluminum. Methods Thirty-five male Wistar rats were randomly divided into five groups： the negative control group,aluminum treated group and AI＋ DFP low,Al＋ DFP moderate and AI＋ DFP high dose groups. The negative control group were administered with NS （1 ml/d） for 10 weeks,the aluminum treated group were administered with 354.7 mg/（kg.d） aluminum chloride for 8 weeks,then treated with NS （1 ml/d） for another 2 weeks.The low, mid, high dose groups （AI＋DEP） were administered with 354.7 mg/（kg, d） aluminum chloride for eight weeks,then treated with different doses of 13.72,27.44 and 54.88 mg/（kg,d） DFP for another two weeks. The drugs were given by gavage,once a day, five days a week. The contents of Gly, GABA and aluminum and essential elements such as copper,magnesium,calcium and iron in brain were determined respectively. Results Compared with negative control group, the content of Gly and GABA increased significantly in aluminum treated group and AI＋ DFP dose groups （P〈0.01）. Meanwhile, there were significant decrease in the content of Gly and GABA in three different AI＋ DFP dose groups than aluminum treated group （P〈0.01）. The content of A1 of low,moderate dose groups （AI＋DFP） were significantly higher than the negative control group（P〈0.01） and the three different dose groups （AI＋DFP） lower than aluminum treated group （P〈0.01）. With the DFP dose increased,the content of A1 in AI＋ DFP dose groups showed a decreasing trend. There were no significant differences among various groups in the contents of copper,magnesium,calcium and iron elements in brain. Conclusion DFP may effectively enhance the elimination of accumulated ：aluminum in the brain,recover the metabolism of Gly and GABA with little effect on the balance of Cu, Mg, Ca and Fe concentrations in the brain, and it may produce a protective effect on central nervous system in rats exposed to aluminum.