辛硫磷对小鼠外周血淋巴细胞DNA损伤的研究

DNA Damage among Peripheral Lymphocytes Induced by Phoxim Exposure in Mice

目的探讨辛硫磷对小鼠的遗传毒性。方法将60只健康SPF级昆明小鼠随机分为6组,分别为阴性对照(生理盐水)组和10、20、40、80 mg/kg辛硫磷染毒组以及阳性对照组(40 mg/kg环磷酰胺腹腔注射,每日1次,连续2 d),每组10只,雌雄各半。采用灌胃方式进行染毒,染毒容量为10 ml/kg,每日1次,连续染毒14 d。采用微核试验和单细胞凝胶电泳试验检测辛硫磷的遗传毒性。结果与阴性对照组比较,仅40和80 mg/kg辛硫磷染毒组小鼠的骨髓细胞微核率显著性升高,各剂量辛硫磷染毒组小鼠外周血淋巴细胞的拖尾率和DNA损伤专用单位均升高,差异有统计学意义(P<0.05)。随着辛硫磷染毒剂量的升高,小鼠骨髓细胞微核率、外周血淋巴细胞的拖尾率和DNA损伤专用单位均呈上升趋势。结论辛硫磷对小鼠具有遗传毒性。

Objective To explore the genotoxicity of phoxim to mice. Methods Sixty healthy SPF grade,KM mice were randomly divided into six groups and were given phoxim by gavage at several doses of 0, 10, 20, 40 and 80 mg/kg bw respectively once a day for fourteen consecutive days. Bone marrow mieronuclei test and single cell gel electrophoresis were employed to test the genotoxicity. Results Compared with the negative control group,a significant increase in the rate of micronuelei at higher doses （40 and 80 mg/kg） and the DNA damage of peripheral lymphoeytes in all experimental groups were found （P〈0.05）. As for the rate of mieronuelei and DNA damage, an obvious dose-effect relationship was seen. Conclusion Phoxim has a genotoxic effect on mice.